Zur Schockdiagnostik: Der Nachweis von Endotoxin und Mucopeptid mit dem Limulus polyphemus-Lysat-Test

Translated title of the contribution: Contribution to the diagnosis of shock: Demonstration of endotoxin and mucopeptide by the limulus polyphemus-lysate test

A. Wildfeuer, B. Heymer, K. H. Schleifer, H. P. Seidel, O. Haferkamp

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Gelation of amebocyte lysate from Limulus polyphemus (LPL) is induced not only by endotoxins of gramnegative bacteria but also by mucopeptides of various grampositive organisms. Solubilization considerably increases mucopeptide LPL-activity. Total activity of mucopeptide, however, is about 1000-100 000 times smaller than that of endotoxin. In contrast to endotoxin, treatment of mucopeptide with lysozyme causes decrease or complete loss of LPL-activity. Also mucopeptide thus handled does not react any more in the capillary precipitin test. The possibility to destroy mucopeptide LPL-activity by pretreatment with lysozyme permits differentiation from LPL-gelation by endotoxin. In blood specimens of patients with suspected endotoxemia and positive LPL-test mucopeptide induced LPL-reaction therefore can be excluded by pretreatment with lysozyme.

Translated title of the contributionContribution to the diagnosis of shock: Demonstration of endotoxin and mucopeptide by the limulus polyphemus-lysate test
Original languageGerman
Pages (from-to)175-178
Number of pages4
JournalKlinische Wochenschrift
Volume52
Issue number4
DOIs
StatePublished - Feb 1974
Externally publishedYes

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