Zum nachweis toxinogener Bacillus cereus-stämme

Translated title of the contribution: Detection of toxinogenic Bacillus cereus

K. E. Seidel, C. Pfisterer, S. Hormansdorfer, J. Bauer

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Investigations into optimal culture conditions for Bacillus cereus in order to detect bacterial toxins in a cell culture system showed that Dulbecco's modified Eagle's medium supplemented with 5 % fetal calf serum is the medium best suited for this purpose. The highest toxicity levels were seen when bacteria were cultured at 21°C. In 42 of 43 Bacillus cereus-strains isolated from milk and milk products, toxin formation was detected using the MTT-test. In 11 strains, toxin formation was even shown when bacteria were cultured at 4°C. When culture supernatants were examined in a commercially available ELISA, all cytotoxic strains were shown to form diarrheal toxin.

Translated title of the contributionDetection of toxinogenic Bacillus cereus
Original languageGerman
Pages (from-to)421-428
Number of pages8
JournalJournal of Veterinary Medicine, Series B
Volume43
Issue number7
DOIs
StatePublished - Sep 1996

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