Zum Nachweis der Shiga-like Toxine von Escherichia coli mit Hilfe des MTT-Zellkulturtests

Translated title of the contribution: Determination of Escherichia coli's Shiga-like toxins by means of the MTT-bioassay

Stefan Hörmansdorfer, Manfred Gareis, Johann Bauer, Anton Mayr

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Tissue culture cells' metabolism and viability are measured by the mitochondrial reduction rate of a yellow tetrazolium salt (MTT) to blue formazan crystalls in the MTT-bioassay. Thus the MTT-bioassay is a standardizable and reproducible bioassay for measuring cytotoxicity or cytostimulation. It is shown that the MTT-bioassay is also very suitable for determining bacterial cytotoxins using Escherichia coli's Shiga-like toxins as example. 177 strains of E.coli, isolated from carcasses and organs of cattle, are classified biochemically and tested for cytotoxin production by means of the MTT-bioassay. One of these strains is recognized as producer of Shiga-like toxin 2. 4 Enterohemolysin-producing strains of E.coli are cultivated from a feces sample of a diarrhoeic nubian ibex and identified as Shiga-like toxin 1 producers by help of the MTT-bioassay.

Translated title of the contributionDetermination of Escherichia coli's Shiga-like toxins by means of the MTT-bioassay
Original languageGerman
Pages (from-to)293-297
Number of pages5
JournalZeitschrift fur Lebensmittel -Untersuchung und -Forschung
Volume201
Issue number3
DOIs
StatePublished - May 1995

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