YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication by inducing E2 gene expression through the E2 late promoter

Per S. Holm, Stephan Bergmann, Karsten Jürchott, Hermann Lage, Karsten Brand, Axel Ladhoff, Klaus Mantwill, David T. Curiel, Matthias Dobbelstein, Manfred Dietel, Bernd Gänsbacher, Hans Dieter Royer

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

The adenovirus early proteins E1A and E1B-55kDa are key regulators of viral DNA replication, and it was thought that targeting of p53 by E1B-55kDa is essential for this process. Here we have identified a previously unrecognized function of E1B for adenovirus replication. We found that E1B-55kDa is involved in targeting the transcription factor YB-1 to the nuclei of adenovirus type 5-infected cells where it is associated with viral inclusion bodies believed to be sites of viral transcription and replication. We show that YB-1 facilitates E2 gene expression through the E2 late promoter thus controlling E2 gene activity at later stages of infection. The role of YB-1 for adenovirus replication was demonstrated with an E1-minus adenovirus vector containing a YB-1 transgene. In infected cells, AdYB-1 efficiently replicated and produced infectious progeny particles. Thus, adenovirus E1B-55kDa protein and the host cell factor YB-1 act jointly to facilitate adenovirus replication in the late phase of infection.

Original languageEnglish
Pages (from-to)10427-10434
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number12
DOIs
StatePublished - 22 Mar 2002
Externally publishedYes

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