TY - JOUR
T1 - X-ray phase tomography with near-field speckles for three-dimensional virtual histology
AU - Zdora, Marie Christine
AU - Thibault, Pierre
AU - Kuo, Willy
AU - Fernandez, Vincent
AU - Deyhle, Hans
AU - Vila-Comamala, Joan
AU - Olbinado, Margie P.
AU - Rack, Alexander
AU - Lackie, Peter M.
AU - Katsamenis, Orestis L.
AU - Lawson, Matthew J.
AU - Kurtcuoglu, Vartan
AU - Rau, Christoph
AU - Pfeiffer, Franz
AU - Zanette, Irene
N1 - Publisher Copyright:
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
PY - 2020/9/20
Y1 - 2020/9/20
N2 - High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2 mg/cm3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.
AB - High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2 mg/cm3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.
UR - https://www.scopus.com/pages/publications/85091042863
U2 - 10.1364/OPTICA.399421
DO - 10.1364/OPTICA.399421
M3 - Article
AN - SCOPUS:85091042863
SN - 2334-2536
VL - 7
SP - 1221
EP - 1227
JO - Optica
JF - Optica
IS - 9
ER -