@article{5298371ea7ff48a68d57878d3d60cbab,
title = "X-ray crystallographic and NMR studies of the third KH domain of hnRNP K in complex with single-stranded nucleic acids",
abstract = "The heterogeneous nuclear ribonucleoprotein (hnRNP) K is implicated in multiple functions in the regulation of gene expression and acts as a hub at the intersection of signaling pathways and processes involving nucleic acids. Central to its function is its ability to bind both ssDNA and ssRNA via its KH (hnRNP K homology) domains. We determined crystal structures of hnRNP K KH3 domain complexed with 15-mer and 6-mer (CTC4) ssDNAs at 2.4 and 1.8 {\AA} resolution, respectively, and show that the KH3 domain binds specifically to both TCCC and CCCC sequences. In parallel, we used NMR to compare the binding affinity and mode of interaction of the KH3 domain with several ssRNA ligands and CTC4 ssDNA. Based on a structure alignment of the KH3-CTC4 complex with known structures of other KH domains in complex with ssRNA, we discuss recognition of tetranucleotide sequences by KH domains.",
author = "Backe, {Paul H.} and Messias, {Ana C.} and Ravelli, {Raimond B.G.} and Michael Sattler and Stephen Cusack",
note = "Funding Information: We thank the European Synchrotron Radiation Facility-European Molecular Biology Laboratory (ESRF-EMBL) Joint Structural Biology Group for access to ESRF beamlines. We are grateful to Dr. Marius Clore (NIH, USA) for sending the experimental NMR data used for the structure determination of the hnRNP K complex with DNA ( Braddock et al., 2002a ). P.H.B. received a European Molecular Biology Laboratory predoctoral fellowship and was also supported by a grant from The Norwegian Research Council. A.C.M. acknowledges SFRH/BPD/14893/2004 and PRAXIS XXI/BPD/22071/99 postdoctoral fellowships. Figures were created with MOLSCRIPT ( Kraulis, 1991 ) or BOBSCRIPT ( Esnouf, 1997 ) and were rendered with RASTER3D ( Merritt and Murphy, 1994 ). ",
year = "2005",
month = jul,
doi = "10.1016/j.str.2005.04.008",
language = "English",
volume = "13",
pages = "1055--1067",
journal = "Structure",
issn = "0969-2126",
publisher = "Cell Press",
number = "7",
}