Volumetric tomography of fluorescent proteins through small animals in vivo

Giannis Zacharakis; Hirokazu Kambara; Helen Shih; Jorge Ripoll; Jan Grimm; Yoshinaga Saeki; Ralph Weissleder; Vasilis Ntziachristos

doi:10.1073/pnas.0504628102

Volumetric tomography of fluorescent proteins through small animals in vivo

Giannis Zacharakis
, Hirokazu Kambara
, Helen Shih
, Jorge Ripoll
, Jan Grimm
, Yoshinaga Saeki
, Ralph Weissleder
, Vasilis Ntziachristos

Massachusetts General Hospital
Foundation for Research and Technology-Hellas, Institute of Electronic Structure and Laser

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

Volumetric detection and accurate quantification of fluorescent proteins in entire animals would greatly enhance our ability to monitor biological processes in vivo. Here we present a quantitative tomographic technique for visualization of superficial and deep-seated (>2-3 mm) fluorescent protein activity in vivo. We demonstrate noninvasive imaging of lung tumor progression in a murine model, as well as imaging of gene delivery using a herpes virus vector. This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research.

Original language	English
Pages (from-to)	18252-18257
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	102
Issue number	51
DOIs	https://doi.org/10.1073/pnas.0504628102
State	Published - 20 Dec 2005
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Fluorescence
Gene transfer
Imaging gene expression
Multispectral imaging
Whole-body imaging

Access to Document

10.1073/pnas.0504628102

Cite this

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title = "Volumetric tomography of fluorescent proteins through small animals in vivo",

abstract = "Volumetric detection and accurate quantification of fluorescent proteins in entire animals would greatly enhance our ability to monitor biological processes in vivo. Here we present a quantitative tomographic technique for visualization of superficial and deep-seated (>2-3 mm) fluorescent protein activity in vivo. We demonstrate noninvasive imaging of lung tumor progression in a murine model, as well as imaging of gene delivery using a herpes virus vector. This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research.",

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T1 - Volumetric tomography of fluorescent proteins through small animals in vivo

AU - Zacharakis, Giannis

AU - Kambara, Hirokazu

AU - Shih, Helen

AU - Ripoll, Jorge

AU - Grimm, Jan

AU - Saeki, Yoshinaga

AU - Weissleder, Ralph

AU - Ntziachristos, Vasilis

PY - 2005/12/20

Y1 - 2005/12/20

N2 - Volumetric detection and accurate quantification of fluorescent proteins in entire animals would greatly enhance our ability to monitor biological processes in vivo. Here we present a quantitative tomographic technique for visualization of superficial and deep-seated (>2-3 mm) fluorescent protein activity in vivo. We demonstrate noninvasive imaging of lung tumor progression in a murine model, as well as imaging of gene delivery using a herpes virus vector. This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research.

AB - Volumetric detection and accurate quantification of fluorescent proteins in entire animals would greatly enhance our ability to monitor biological processes in vivo. Here we present a quantitative tomographic technique for visualization of superficial and deep-seated (>2-3 mm) fluorescent protein activity in vivo. We demonstrate noninvasive imaging of lung tumor progression in a murine model, as well as imaging of gene delivery using a herpes virus vector. This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research.

KW - Fluorescence

KW - Gene transfer

KW - Imaging gene expression

KW - Multispectral imaging

KW - Whole-body imaging

UR - https://www.scopus.com/pages/publications/29444437303

U2 - 10.1073/pnas.0504628102

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AN - SCOPUS:29444437303

SN - 0027-8424

VL - 102

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 51

ER -

Volumetric tomography of fluorescent proteins through small animals in vivo

Abstract

UN SDGs

Keywords

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