Viable pigs with a conditionally-activated oncogenic KRAS mutation

Shun Li, Marlene Edlinger, Anja Saalfrank, Krzysztof Flisikowski, Alexander Tschukes, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Dieter Saur, Alexander Kind, Eckhard Wolf, Angelika Schnieke, Tatiana Flisikowska

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Oncogenic mutations of KRAS play a major role in human carcinogenesis. Here we describe viable gene-targeted pigs carrying a latent KRASG12D mutant allele that can be activated by Cre recombination. These have been produced as part of a program to model human cancers in pigs by replicating genetic lesions known to initiate and drive human disease. Cre-activated KRASG12D animals add to a growing set of gene-targeted pigs that includes a Cre-activated oncogenic mutant TP53, a Cre-responsive dual fluorescent reporter and two truncating mutations of APC (adenomatous polyposis coli). These alleles can be combined and activated in various tissues to produce new models for cancer research.

Original languageEnglish
Pages (from-to)509-517
Number of pages9
JournalTransgenic Research
Volume24
Issue number3
DOIs
StatePublished - 26 Jun 2015

Keywords

  • Cancer
  • Conditional gene activation
  • Cre recombination
  • Disease model
  • KRAS
  • Swine

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