Viable pigs with a conditionally-activated oncogenic KRAS mutation

Shun Li; Marlene Edlinger; Anja Saalfrank; Krzysztof Flisikowski; Alexander Tschukes; Mayuko Kurome; Valeri Zakhartchenko; Barbara Kessler; Dieter Saur; Alexander Kind; Eckhard Wolf; Angelika Schnieke; Tatiana Flisikowska

doi:10.1007/s11248-015-9866-8

Viable pigs with a conditionally-activated oncogenic KRAS mutation

Shun Li, Marlene Edlinger, Anja Saalfrank, Krzysztof Flisikowski, Alexander Tschukes, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Dieter Saur, Alexander Kind, Eckhard Wolf, Angelika Schnieke, Tatiana Flisikowska

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27 Scopus citations

Abstract

Oncogenic mutations of KRAS play a major role in human carcinogenesis. Here we describe viable gene-targeted pigs carrying a latent KRAS^G12D mutant allele that can be activated by Cre recombination. These have been produced as part of a program to model human cancers in pigs by replicating genetic lesions known to initiate and drive human disease. Cre-activated KRAS^G12D animals add to a growing set of gene-targeted pigs that includes a Cre-activated oncogenic mutant TP53, a Cre-responsive dual fluorescent reporter and two truncating mutations of APC (adenomatous polyposis coli). These alleles can be combined and activated in various tissues to produce new models for cancer research.

Original language	English
Pages (from-to)	509-517
Number of pages	9
Journal	Transgenic Research
Volume	24
Issue number	3
DOIs	https://doi.org/10.1007/s11248-015-9866-8
State	Published - 26 Jun 2015

Keywords

Cancer
Conditional gene activation
Cre recombination
Disease model
KRAS
Swine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11248-015-9866-8

Cite this

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title = "Viable pigs with a conditionally-activated oncogenic KRAS mutation",

abstract = "Oncogenic mutations of KRAS play a major role in human carcinogenesis. Here we describe viable gene-targeted pigs carrying a latent KRASG12D mutant allele that can be activated by Cre recombination. These have been produced as part of a program to model human cancers in pigs by replicating genetic lesions known to initiate and drive human disease. Cre-activated KRASG12D animals add to a growing set of gene-targeted pigs that includes a Cre-activated oncogenic mutant TP53, a Cre-responsive dual fluorescent reporter and two truncating mutations of APC (adenomatous polyposis coli). These alleles can be combined and activated in various tissues to produce new models for cancer research.",

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author = "Shun Li and Marlene Edlinger and Anja Saalfrank and Krzysztof Flisikowski and Alexander Tschukes and Mayuko Kurome and Valeri Zakhartchenko and Barbara Kessler and Dieter Saur and Alexander Kind and Eckhard Wolf and Angelika Schnieke and Tatiana Flisikowska",

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T1 - Viable pigs with a conditionally-activated oncogenic KRAS mutation

AU - Li, Shun

AU - Edlinger, Marlene

AU - Saalfrank, Anja

AU - Flisikowski, Krzysztof

AU - Tschukes, Alexander

AU - Kurome, Mayuko

AU - Zakhartchenko, Valeri

AU - Kessler, Barbara

AU - Saur, Dieter

AU - Kind, Alexander

AU - Wolf, Eckhard

AU - Schnieke, Angelika

AU - Flisikowska, Tatiana

PY - 2015/6/26

Y1 - 2015/6/26

N2 - Oncogenic mutations of KRAS play a major role in human carcinogenesis. Here we describe viable gene-targeted pigs carrying a latent KRASG12D mutant allele that can be activated by Cre recombination. These have been produced as part of a program to model human cancers in pigs by replicating genetic lesions known to initiate and drive human disease. Cre-activated KRASG12D animals add to a growing set of gene-targeted pigs that includes a Cre-activated oncogenic mutant TP53, a Cre-responsive dual fluorescent reporter and two truncating mutations of APC (adenomatous polyposis coli). These alleles can be combined and activated in various tissues to produce new models for cancer research.

AB - Oncogenic mutations of KRAS play a major role in human carcinogenesis. Here we describe viable gene-targeted pigs carrying a latent KRASG12D mutant allele that can be activated by Cre recombination. These have been produced as part of a program to model human cancers in pigs by replicating genetic lesions known to initiate and drive human disease. Cre-activated KRASG12D animals add to a growing set of gene-targeted pigs that includes a Cre-activated oncogenic mutant TP53, a Cre-responsive dual fluorescent reporter and two truncating mutations of APC (adenomatous polyposis coli). These alleles can be combined and activated in various tissues to produce new models for cancer research.

KW - Cancer

KW - Conditional gene activation

KW - Cre recombination

KW - Disease model

KW - KRAS

KW - Swine

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SP - 509

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JF - Transgenic Research

IS - 3

ER -

Viable pigs with a conditionally-activated oncogenic KRAS mutation

Abstract

Keywords

UN SDGs

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