TY - JOUR
T1 - Verbascum nigrum
T2 - Cytotoxicity Evaluation in A431 Epidermoid Carcinoma Cells and Untargeted LC-HR-MS/MS Metabolite Profiling
AU - Iliescu, Iolanda Alca
AU - Peter, Samuel
AU - Albert, Ina
AU - Skalicka-Woźniak, Krystyna
AU - Miron, Anca
AU - Luca, Simon Vlad
AU - Wolfram, Evelyn
N1 - Publisher Copyright:
© 2020 The Authors. Chemistry & Biodiversity published by Wiley-VHCA AG, Zurich, Switzerland
PY - 2020/12
Y1 - 2020/12
N2 - The crude methanolic extract obtained from Verbascum nigrum aerial parts (VNE) and its six fractions (VNF1–VNF6) were initially screened regarding their effects on the viability of immortalized HaCaT keratinocytes and A431 epidermoid carcinoma cells (MTT assay, 24 h). None of the tested samples affected the viability of HaCaT cells in a concentration range of 25–150 μg/mL. VNE and VNF4 exhibited significant cytotoxic effects in A431 cells, with IC50 values of 81.92 and 12.27 μg/mL, respectively; the selectivity index was higher than 10 for VNF4. The untargeted LC/HR-MS/MS metabolite profiling led to the tentative annotation of a total number of 23 compounds. Of these, VNE comprised mainly iridoid glycosides (harpagoside, laterioside, acylated aucubin derivatives), whereas VNF4 showed a high abundance of triterpene saponin glycosides (ilwensisaponins A and C, songarosaponins A and B), constituents known for their selective cytotoxic potential.
AB - The crude methanolic extract obtained from Verbascum nigrum aerial parts (VNE) and its six fractions (VNF1–VNF6) were initially screened regarding their effects on the viability of immortalized HaCaT keratinocytes and A431 epidermoid carcinoma cells (MTT assay, 24 h). None of the tested samples affected the viability of HaCaT cells in a concentration range of 25–150 μg/mL. VNE and VNF4 exhibited significant cytotoxic effects in A431 cells, with IC50 values of 81.92 and 12.27 μg/mL, respectively; the selectivity index was higher than 10 for VNF4. The untargeted LC/HR-MS/MS metabolite profiling led to the tentative annotation of a total number of 23 compounds. Of these, VNE comprised mainly iridoid glycosides (harpagoside, laterioside, acylated aucubin derivatives), whereas VNF4 showed a high abundance of triterpene saponin glycosides (ilwensisaponins A and C, songarosaponins A and B), constituents known for their selective cytotoxic potential.
KW - cytotoxic activity
KW - LC/HR-MS/MS
KW - saponin glycosides
KW - verbascoside
KW - Verbascum nigrum
UR - http://www.scopus.com/inward/record.url?scp=85097019121&partnerID=8YFLogxK
U2 - 10.1002/cbdv.202000644
DO - 10.1002/cbdv.202000644
M3 - Article
C2 - 33119147
AN - SCOPUS:85097019121
SN - 1612-1872
VL - 17
JO - Chemistry and Biodiversity
JF - Chemistry and Biodiversity
IS - 12
M1 - e2000644
ER -