TY - JOUR
T1 - Vasorelaxant effects of Crataegus pentagyna
T2 - Links with arginase inhibition and phenolic profile
AU - Bujor, Alexandra
AU - Miron, Anca
AU - Luca, Simon Vlad
AU - Skalicka-Wozniak, Krystyna
AU - Silion, Mihaela
AU - Trifan, Adriana
AU - Girard, Corine
AU - Demougeot, Céline
AU - Totoson, Perle
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/4/24
Y1 - 2020/4/24
N2 - Ethnopharmacological relevance: Crataegus leaves, flowers and fruits have been traditionally used to improve blood circulation, numerous preclinical and clinical studies supporting the cardiovascular benefits of Crataegus preparations. In this respect, there is very limited data on Crataegus pentagyna; in addition, the chemical profile of this species is still incompletely elucidated. Aim of the study: The objective of this study was to examine the cardiovascular benefits of Crataegus pentagyna Waldst. et Kit. ex Willd. (small-flowered black hawthorn, Rosaceae) extracts (leaf, flower and fruit ethyl acetate extracts) and the underlying mechanisms. We hypothesized that C. pentagyna extracts might exert vasodilatory effects and inhibit arginase activity due, in large part, to their polyphenolic constituents. Materials and methods: C. pentagyna extracts induced-relaxation and the mechanisms involved were studied ex vivo in isolated aortic rings from Sprague-Dawley rats. The inhibitory effects on bovine liver arginase I were assessed by an in vitro assay. Metabolite profiling of C. pentagyna extracts was performed and the most endothelium- and nitric oxide synthase-dependent; flower extract additionally reduced Ca2+ entry and, to a lesser extent, Ca2+ release from the sarcoplasmic reticulum. C. pentagyna proved to be an important source of arginase inhibitors with potential benefits in endothelial dysfunction that remains to be explored.
AB - Ethnopharmacological relevance: Crataegus leaves, flowers and fruits have been traditionally used to improve blood circulation, numerous preclinical and clinical studies supporting the cardiovascular benefits of Crataegus preparations. In this respect, there is very limited data on Crataegus pentagyna; in addition, the chemical profile of this species is still incompletely elucidated. Aim of the study: The objective of this study was to examine the cardiovascular benefits of Crataegus pentagyna Waldst. et Kit. ex Willd. (small-flowered black hawthorn, Rosaceae) extracts (leaf, flower and fruit ethyl acetate extracts) and the underlying mechanisms. We hypothesized that C. pentagyna extracts might exert vasodilatory effects and inhibit arginase activity due, in large part, to their polyphenolic constituents. Materials and methods: C. pentagyna extracts induced-relaxation and the mechanisms involved were studied ex vivo in isolated aortic rings from Sprague-Dawley rats. The inhibitory effects on bovine liver arginase I were assessed by an in vitro assay. Metabolite profiling of C. pentagyna extracts was performed and the most endothelium- and nitric oxide synthase-dependent; flower extract additionally reduced Ca2+ entry and, to a lesser extent, Ca2+ release from the sarcoplasmic reticulum. C. pentagyna proved to be an important source of arginase inhibitors with potential benefits in endothelial dysfunction that remains to be explored.
KW - Aortic rings
KW - Arginase inhibition
KW - Caffeic acid (PubChem CID: 689043)
KW - Chlorogenic acid (PubChem CID: 1794427)
KW - Citric acid (PubChem CID: 311)
KW - Crataegus pentagyna Waldst. et Kit. ex Willd.
KW - Epicatechin (PubChem CID: 1203)
KW - Neochlorogenic acid (PubChem CID: 5280633)
KW - Nor-NOHA, Nω-hydroxy-nor-arginine (PubChem CID: 446124)
KW - Polyphenolic compounds
KW - Quercetin (PubChem CID: 5280343)
KW - Rutin (PubChem CID: 5280805)
KW - Vasorelaxation
UR - http://www.scopus.com/inward/record.url?scp=85078017529&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2020.112559
DO - 10.1016/j.jep.2020.112559
M3 - Article
C2 - 31935497
AN - SCOPUS:85078017529
SN - 0378-8741
VL - 252
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
M1 - 112559
ER -