Variability of protein and phosphoprotein levels in clinical tissue specimens during the preanalytical phase

Sibylle Gündisch, Stefanie Hauck, Hakan Sarioglu, Christina Schott, Christian Viertler, Marcel Kap, Tibor Schuster, Bilge Reischauer, Robert Rosenberg, Cornelis Verhoef, Hans Joerg Mischinger, Peter Riegman, Kurt Zatloukal, Karl Friedrich Becker

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The quality of human tissue specimens can have a significant impact on analytical data sets for biomarker research. The aim of this study was to characterize fluctuations of protein and phosphoprotein levels in human tissue samples during the preanalytical phase. Eleven intestine and 17 liver specimens were surgically resected, aliquoted, and either snap-frozen or fixed in formalin immediately or exposed to different ischemic conditions before preservation. Protein levels in the resultant samples were investigated by reverse phase protein array, Western blot analysis, and liquid chromatography-tandem mass spectrometry. Our data revealed that the degree of sensitivity of proteins and phosphoproteins to delayed preservation varied between different patients and tissue types. For example, up-regulation of phospho-p42/44 MAPK in intestine samples was seen in some patients but not in others. General trends toward up- or down-regulation of most proteins were not evident due to pronounced interpatient variability but signal intensities of only a few proteins, such as cytokeratin 18, were altered from baseline in postresection samples. In contrast, glyceraldehyde 3-phosphate dehydrogenase was found to be stable during periods of cold ischemia. Our study represents a proper approach for studying potential protein fluctuations in tissue specimens for future biomarker development programs.

Original languageEnglish
Pages (from-to)5748-5762
Number of pages15
JournalJournal of Proteome Research
Volume11
Issue number12
DOIs
StatePublished - 7 Dec 2012

Keywords

  • biobank
  • biomarker
  • preanalytical phase
  • protein
  • tissue

Fingerprint

Dive into the research topics of 'Variability of protein and phosphoprotein levels in clinical tissue specimens during the preanalytical phase'. Together they form a unique fingerprint.

Cite this