TY - JOUR
T1 - Validation of the use of a fluorescent PARP1 inhibitor for the detection of oral, oropharyngeal and oesophageal epithelial cancers
AU - Kossatz, Susanne
AU - Pirovano, Giacomo
AU - Demétrio De Souza França, Paula
AU - Strome, Arianna L.
AU - Sunny, Sumsum P.
AU - Zanoni, Daniella Karassawa
AU - Mauguen, Audrey
AU - Carney, Brandon
AU - Brand, Christian
AU - Shah, Veer
AU - Ramanajinappa, Ravindra D.
AU - Hedne, Naveen
AU - Birur, Praveen
AU - Sihag, Smita
AU - Ghossein, Ronald A.
AU - Gönen, Mithat
AU - Strome, Marshall
AU - Suresh, Amritha
AU - Molena, Daniela
AU - Ganly, Ian
AU - Kuriakose, Moni A.
AU - Patel, Snehal G.
AU - Reiner, Thomas
N1 - Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - For oral, oropharyngeal and oesophageal cancer, the early detection of tumours and of residual tumour after surgery are prognostic factors of recurrence rates and patient survival. Here, we report the validation, in animal models and a human, of the use of a previously described fluorescently labelled small-molecule inhibitor of the DNA repair enzyme poly(ADP–ribose) polymerase 1 (PARP1) for the detection of cancers of the oral cavity, pharynx and oesophagus. We show that the fluorescent contrast agent can be used to quantify the expression levels of PARP1 and to detect oral, oropharyngeal and oesophageal tumours in mice, pigs and fresh human biospecimens when delivered topically or intravenously. The fluorescent PARP1 inhibitor can also detect oral carcinoma in a patient when applied as a mouthwash, and discriminate between fresh biopsied samples of the oral tumour and the surgical resection margin with more than 95% sensitivity and specificity. The PARP1 inhibitor could serve as the basis of a rapid and sensitive assay for the early detection and for the surgical-margin assessment of epithelial cancers of the upper intestinal tract.
AB - For oral, oropharyngeal and oesophageal cancer, the early detection of tumours and of residual tumour after surgery are prognostic factors of recurrence rates and patient survival. Here, we report the validation, in animal models and a human, of the use of a previously described fluorescently labelled small-molecule inhibitor of the DNA repair enzyme poly(ADP–ribose) polymerase 1 (PARP1) for the detection of cancers of the oral cavity, pharynx and oesophagus. We show that the fluorescent contrast agent can be used to quantify the expression levels of PARP1 and to detect oral, oropharyngeal and oesophageal tumours in mice, pigs and fresh human biospecimens when delivered topically or intravenously. The fluorescent PARP1 inhibitor can also detect oral carcinoma in a patient when applied as a mouthwash, and discriminate between fresh biopsied samples of the oral tumour and the surgical resection margin with more than 95% sensitivity and specificity. The PARP1 inhibitor could serve as the basis of a rapid and sensitive assay for the early detection and for the surgical-margin assessment of epithelial cancers of the upper intestinal tract.
UR - http://www.scopus.com/inward/record.url?scp=85081720833&partnerID=8YFLogxK
U2 - 10.1038/s41551-020-0526-9
DO - 10.1038/s41551-020-0526-9
M3 - Article
C2 - 32165735
AN - SCOPUS:85081720833
SN - 2157-846X
VL - 4
SP - 272
EP - 285
JO - Nature Biomedical Engineering
JF - Nature Biomedical Engineering
IS - 3
ER -