TY - JOUR
T1 - Validation of Arxula Yeast Estrogen Screen assay for detection of estrogenic activity in water samples
T2 - Results of an international interlaboratory study
AU - Hettwer, Karina
AU - Jähne, Martin
AU - Frost, Kirstin
AU - Giersberg, Martin
AU - Kunze, Gotthard
AU - Trimborn, Michael
AU - Reif, Martin
AU - Türk, Jochen
AU - Gehrmann, Linda
AU - Dardenne, Freddy
AU - De Croock, Femke
AU - Abraham, Marion
AU - Schoop, Anne
AU - Waniek, Joanna J.
AU - Bucher, Thomas
AU - Simon, Eszter
AU - Vermeirssen, Etienne
AU - Werner, Anett
AU - Hellauer, Karin
AU - Wallentits, Ursula
AU - Drewes, Jörg E.
AU - Dietzmann, Detlef
AU - Routledge, Edwin
AU - Beresford, Nicola
AU - Zietek, Tamara
AU - Siebler, Margot
AU - Simon, Anne
AU - Bielak, Helena
AU - Hollert, Henner
AU - Müller, Yvonne
AU - Harff, Maike
AU - Schiwy, Sabrina
AU - Simon, Kirsten
AU - Uhlig, Steffen
N1 - Publisher Copyright:
© 2017
PY - 2018/4/15
Y1 - 2018/4/15
N2 - Endocrine-active substances can adversely impact the aquatic ecosystems. A special emphasis is laid, among others, on the effects of estrogens and estrogen mimicking compounds. Effect-based screening methods like in vitro bioassays are suitable tools to detect and quantify endocrine activities of known and unknown mixtures. This study describes the validation of the Arxula-Yeast Estrogen Screen (A-YES®) assay, an effect-based method for the detection of the estrogenic potential of water and waste water. This reporter gene assay, provided in ready to use format, is based on the activation of the human estrogen receptor alpha. The user-friendly A-YES® enables inexperienced operators to rapidly become competent with the assay. Fourteen laboratories from four countries with different training levels analyzed 17β-estradiol equivalent concentrations (EEQ) in spiked and unspiked waste water effluent and surface water samples, in waste water influent and spiked salt water samples and in a mixture of three bisphenols. The limit of detection (LOD) for untreated samples was 1.8 ng/L 17β-estradiol (E2). Relative repeatability and reproducibility standard deviation for samples with EEQ above the LOD (mean EEQ values between 6.3 and 20.4 ng/L) ranged from 7.5 to 21.4% and 16.6 to 28.0%, respectively. Precision results are comparable to other frequently used analytical methods for estrogens. The A-YES® has been demonstrated to be an accurate, precise and robust bioassay. The results have been included in the ISO draft standard. The assay was shown to be applicable for testing of typical waste water influent, effluent and saline water. Other studies have shown that the assay can be used with enriched samples, which lower the LOD to the pg/L range. The validation of the A-YES® and the development of a corresponding international standard constitute a step further towards harmonized and reliable bioassays for the effect-based analysis of estrogens and estrogen-like compounds in water samples.
AB - Endocrine-active substances can adversely impact the aquatic ecosystems. A special emphasis is laid, among others, on the effects of estrogens and estrogen mimicking compounds. Effect-based screening methods like in vitro bioassays are suitable tools to detect and quantify endocrine activities of known and unknown mixtures. This study describes the validation of the Arxula-Yeast Estrogen Screen (A-YES®) assay, an effect-based method for the detection of the estrogenic potential of water and waste water. This reporter gene assay, provided in ready to use format, is based on the activation of the human estrogen receptor alpha. The user-friendly A-YES® enables inexperienced operators to rapidly become competent with the assay. Fourteen laboratories from four countries with different training levels analyzed 17β-estradiol equivalent concentrations (EEQ) in spiked and unspiked waste water effluent and surface water samples, in waste water influent and spiked salt water samples and in a mixture of three bisphenols. The limit of detection (LOD) for untreated samples was 1.8 ng/L 17β-estradiol (E2). Relative repeatability and reproducibility standard deviation for samples with EEQ above the LOD (mean EEQ values between 6.3 and 20.4 ng/L) ranged from 7.5 to 21.4% and 16.6 to 28.0%, respectively. Precision results are comparable to other frequently used analytical methods for estrogens. The A-YES® has been demonstrated to be an accurate, precise and robust bioassay. The results have been included in the ISO draft standard. The assay was shown to be applicable for testing of typical waste water influent, effluent and saline water. Other studies have shown that the assay can be used with enriched samples, which lower the LOD to the pg/L range. The validation of the A-YES® and the development of a corresponding international standard constitute a step further towards harmonized and reliable bioassays for the effect-based analysis of estrogens and estrogen-like compounds in water samples.
KW - Effect-based monitoring tools
KW - Estrogenicity
KW - ISO
KW - Interlaboratory trial
KW - Standardization
KW - Yeast Estrogen Screen
UR - http://www.scopus.com/inward/record.url?scp=85035790467&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2017.11.211
DO - 10.1016/j.scitotenv.2017.11.211
M3 - Article
C2 - 29195208
AN - SCOPUS:85035790467
SN - 0048-9697
VL - 621
SP - 612
EP - 625
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -