Validation of Arxula Yeast Estrogen Screen assay for detection of estrogenic activity in water samples: Results of an international interlaboratory study

Karina Hettwer, Martin Jähne, Kirstin Frost, Martin Giersberg, Gotthard Kunze, Michael Trimborn, Martin Reif, Jochen Türk, Linda Gehrmann, Freddy Dardenne, Femke De Croock, Marion Abraham, Anne Schoop, Joanna J. Waniek, Thomas Bucher, Eszter Simon, Etienne Vermeirssen, Anett Werner, Karin Hellauer, Ursula WallentitsJörg E. Drewes, Detlef Dietzmann, Edwin Routledge, Nicola Beresford, Tamara Zietek, Margot Siebler, Anne Simon, Helena Bielak, Henner Hollert, Yvonne Müller, Maike Harff, Sabrina Schiwy, Kirsten Simon, Steffen Uhlig

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Endocrine-active substances can adversely impact the aquatic ecosystems. A special emphasis is laid, among others, on the effects of estrogens and estrogen mimicking compounds. Effect-based screening methods like in vitro bioassays are suitable tools to detect and quantify endocrine activities of known and unknown mixtures. This study describes the validation of the Arxula-Yeast Estrogen Screen (A-YES®) assay, an effect-based method for the detection of the estrogenic potential of water and waste water. This reporter gene assay, provided in ready to use format, is based on the activation of the human estrogen receptor alpha. The user-friendly A-YES® enables inexperienced operators to rapidly become competent with the assay. Fourteen laboratories from four countries with different training levels analyzed 17β-estradiol equivalent concentrations (EEQ) in spiked and unspiked waste water effluent and surface water samples, in waste water influent and spiked salt water samples and in a mixture of three bisphenols. The limit of detection (LOD) for untreated samples was 1.8 ng/L 17β-estradiol (E2). Relative repeatability and reproducibility standard deviation for samples with EEQ above the LOD (mean EEQ values between 6.3 and 20.4 ng/L) ranged from 7.5 to 21.4% and 16.6 to 28.0%, respectively. Precision results are comparable to other frequently used analytical methods for estrogens. The A-YES® has been demonstrated to be an accurate, precise and robust bioassay. The results have been included in the ISO draft standard. The assay was shown to be applicable for testing of typical waste water influent, effluent and saline water. Other studies have shown that the assay can be used with enriched samples, which lower the LOD to the pg/L range. The validation of the A-YES® and the development of a corresponding international standard constitute a step further towards harmonized and reliable bioassays for the effect-based analysis of estrogens and estrogen-like compounds in water samples.

Original languageEnglish
Pages (from-to)612-625
Number of pages14
JournalScience of the Total Environment
Volume621
DOIs
StatePublished - 15 Apr 2018

Keywords

  • Effect-based monitoring tools
  • Estrogenicity
  • ISO
  • Interlaboratory trial
  • Standardization
  • Yeast Estrogen Screen

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