Using Steady-State Fluorescence Anisotropy to Study Protein Clustering

Ajeet Chaudhary, Kay Schneitz

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations


Signaling pathways rely on the precise control of protein-protein interactions. Therefore, it is essential to be able to investigate such interactions with spatiotemporal resolution and in live cells. Here we describe a microscope-based fluorescence spectrometry technique to investigate homotypic interactions between GFP-labeled fusion proteins in a rapid and reproducible fashion using fluorescence anisotropy. This method is of great value for the study of protein complexes in live tissue with subcellular resolution.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Arabidopsis
  • Cell wall
  • Confocal laser scanning microscopy
  • Fluorescence anisotropy
  • Methods
  • Microscopy
  • Plasmodesmata
  • Protein–protein interaction
  • Receptor kinase


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