@inbook{779fbad2d18343c195dfed2545235636,
title = "Using Steady-State Fluorescence Anisotropy to Study Protein Clustering",
abstract = "Signaling pathways rely on the precise control of protein-protein interactions. Therefore, it is essential to be able to investigate such interactions with spatiotemporal resolution and in live cells. Here we describe a microscope-based fluorescence spectrometry technique to investigate homotypic interactions between GFP-labeled fusion proteins in a rapid and reproducible fashion using fluorescence anisotropy. This method is of great value for the study of protein complexes in live tissue with subcellular resolution.",
keywords = "Arabidopsis, Cell wall, Confocal laser scanning microscopy, Fluorescence anisotropy, Methods, Microscopy, Plasmodesmata, Protein–protein interaction, Receptor kinase",
author = "Ajeet Chaudhary and Kay Schneitz",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2132-5_16",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "253--260",
booktitle = "Methods in Molecular Biology",
}