Usefulness of (1,3) ß-d-glucan detection in bronchoalveolar lavage samples in Pneumocystis pneumonia and Pneumocystis pulmonary colonization

C. Damiani; S. Le Gal; N. Goin; P. Di Pizio; C. Da Costa; M. Virmaux; V. Bach; E. Stéphan-Blanchard; G. Nevez; A. Totet

doi:10.1016/j.mycmed.2014.11.001

Usefulness of (1,3) ß-d-glucan detection in bronchoalveolar lavage samples in Pneumocystis pneumonia and Pneumocystis pulmonary colonization

C. Damiani
, S. Le Gal
, N. Goin
, P. Di Pizio
, C. Da Costa
, M. Virmaux
, V. Bach
, E. Stéphan-Blanchard
, G. Nevez
, A. Totet

CHU Sud
Université de Picardie Jules-Verne
Service de Génétique
Université européenne de Bretagne

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Objective of the study: Recent data demonstrate the usefulness of (1,3) ß-. d-glucan (BG) detection in serum samples to distinguish patients developing Pneumocystis pneumonia and patients who are colonized by the fungus. In contrast, data of BG detection in bronchoalveolar lavage (BAL) samples from these patient populations are still rare. Patients: In this context, we determined BG levels in BAL samples from 11 Pneumocystis pneumonia (PCP) patients, 10 colonized patients, and 24 Pneumocystis-uninfected patients. Materials and methods: BG levels were determined on each BAL sample using the Fungitell^® kit (Associates of Cape Cod, Inc., Cape Cod, MA, USA) according to the manufacturer's instructions applied to serum sample examination. Results: The BG levels in BAL samples from the PCP patient group (mean value 20588. pg/mL) were significantly higher than those in the colonized patient group (mean value 105. pg/mL) (P=. 0.0001, Mann-Whitney test) and than those in the Pneumocystis-uninfected patient group (mean value 74. pg/mL) (P<. 0.0001, Mann-Whitney test). The BG levels in BAL samples from the colonized patient group did not differ significantly from those in the Pneumocystis-uninfected patients group (P=. 0.21). Conclusion: The results suggest that measurements of BAL BG levels may facilitate the differential diagnosis of PCP and pulmonary colonization with Pneumocystis.

Original language	English
Pages (from-to)	36-43
Number of pages	8
Journal	Journal de Mycologie Medicale
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/j.mycmed.2014.11.001
State	Published - 1 Mar 2015
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

(1,3) ß-d-glucan
Bronchoalveolar lavage samples
Pneumocystis pneumonia
Pneumocystis pulmonary colonization

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10.1016/j.mycmed.2014.11.001

Cite this

Damiani, C., Le Gal, S., Goin, N., Di Pizio, P., Da Costa, C., Virmaux, M., Bach, V., Stéphan-Blanchard, E., Nevez, G., & Totet, A. (2015). Usefulness of (1,3) ß-d-glucan detection in bronchoalveolar lavage samples in Pneumocystis pneumonia and Pneumocystis pulmonary colonization. Journal de Mycologie Medicale, 25(1), 36-43. https://doi.org/10.1016/j.mycmed.2014.11.001

@article{84260ace80804b77a87a4abba3962e26,

title = "Usefulness of (1,3) {\ss}-d-glucan detection in bronchoalveolar lavage samples in Pneumocystis pneumonia and Pneumocystis pulmonary colonization",

abstract = "Objective of the study: Recent data demonstrate the usefulness of (1,3) {\ss}-. d-glucan (BG) detection in serum samples to distinguish patients developing Pneumocystis pneumonia and patients who are colonized by the fungus. In contrast, data of BG detection in bronchoalveolar lavage (BAL) samples from these patient populations are still rare. Patients: In this context, we determined BG levels in BAL samples from 11 Pneumocystis pneumonia (PCP) patients, 10 colonized patients, and 24 Pneumocystis-uninfected patients. Materials and methods: BG levels were determined on each BAL sample using the Fungitell{\textregistered} kit (Associates of Cape Cod, Inc., Cape Cod, MA, USA) according to the manufacturer's instructions applied to serum sample examination. Results: The BG levels in BAL samples from the PCP patient group (mean value 20588. pg/mL) were significantly higher than those in the colonized patient group (mean value 105. pg/mL) (P=. 0.0001, Mann-Whitney test) and than those in the Pneumocystis-uninfected patient group (mean value 74. pg/mL) (P<. 0.0001, Mann-Whitney test). The BG levels in BAL samples from the colonized patient group did not differ significantly from those in the Pneumocystis-uninfected patients group (P=. 0.21). Conclusion: The results suggest that measurements of BAL BG levels may facilitate the differential diagnosis of PCP and pulmonary colonization with Pneumocystis.",

keywords = "(1,3) {\ss}-d-glucan, Bronchoalveolar lavage samples, Pneumocystis pneumonia, Pneumocystis pulmonary colonization",

author = "C. Damiani and \{Le Gal\}, S. and N. Goin and \{Di Pizio\}, P. and \{Da Costa\}, C. and M. Virmaux and V. Bach and E. St{\'e}phan-Blanchard and G. Nevez and A. Totet",

note = "Publisher Copyright: {\textcopyright} 2014 Elsevier Masson SAS.",

year = "2015",

month = mar,

day = "1",

doi = "10.1016/j.mycmed.2014.11.001",

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Damiani, C, Le Gal, S, Goin, N, Di Pizio, P, Da Costa, C, Virmaux, M, Bach, V, Stéphan-Blanchard, E, Nevez, G & Totet, A 2015, 'Usefulness of (1,3) ß-d-glucan detection in bronchoalveolar lavage samples in Pneumocystis pneumonia and Pneumocystis pulmonary colonization', Journal de Mycologie Medicale, vol. 25, no. 1, pp. 36-43. https://doi.org/10.1016/j.mycmed.2014.11.001

TY - JOUR

T1 - Usefulness of (1,3) ß-d-glucan detection in bronchoalveolar lavage samples in Pneumocystis pneumonia and Pneumocystis pulmonary colonization

AU - Damiani, C.

AU - Le Gal, S.

AU - Goin, N.

AU - Di Pizio, P.

AU - Da Costa, C.

AU - Virmaux, M.

AU - Bach, V.

AU - Stéphan-Blanchard, E.

AU - Nevez, G.

AU - Totet, A.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Objective of the study: Recent data demonstrate the usefulness of (1,3) ß-. d-glucan (BG) detection in serum samples to distinguish patients developing Pneumocystis pneumonia and patients who are colonized by the fungus. In contrast, data of BG detection in bronchoalveolar lavage (BAL) samples from these patient populations are still rare. Patients: In this context, we determined BG levels in BAL samples from 11 Pneumocystis pneumonia (PCP) patients, 10 colonized patients, and 24 Pneumocystis-uninfected patients. Materials and methods: BG levels were determined on each BAL sample using the Fungitell® kit (Associates of Cape Cod, Inc., Cape Cod, MA, USA) according to the manufacturer's instructions applied to serum sample examination. Results: The BG levels in BAL samples from the PCP patient group (mean value 20588. pg/mL) were significantly higher than those in the colonized patient group (mean value 105. pg/mL) (P=. 0.0001, Mann-Whitney test) and than those in the Pneumocystis-uninfected patient group (mean value 74. pg/mL) (P<. 0.0001, Mann-Whitney test). The BG levels in BAL samples from the colonized patient group did not differ significantly from those in the Pneumocystis-uninfected patients group (P=. 0.21). Conclusion: The results suggest that measurements of BAL BG levels may facilitate the differential diagnosis of PCP and pulmonary colonization with Pneumocystis.

AB - Objective of the study: Recent data demonstrate the usefulness of (1,3) ß-. d-glucan (BG) detection in serum samples to distinguish patients developing Pneumocystis pneumonia and patients who are colonized by the fungus. In contrast, data of BG detection in bronchoalveolar lavage (BAL) samples from these patient populations are still rare. Patients: In this context, we determined BG levels in BAL samples from 11 Pneumocystis pneumonia (PCP) patients, 10 colonized patients, and 24 Pneumocystis-uninfected patients. Materials and methods: BG levels were determined on each BAL sample using the Fungitell® kit (Associates of Cape Cod, Inc., Cape Cod, MA, USA) according to the manufacturer's instructions applied to serum sample examination. Results: The BG levels in BAL samples from the PCP patient group (mean value 20588. pg/mL) were significantly higher than those in the colonized patient group (mean value 105. pg/mL) (P=. 0.0001, Mann-Whitney test) and than those in the Pneumocystis-uninfected patient group (mean value 74. pg/mL) (P<. 0.0001, Mann-Whitney test). The BG levels in BAL samples from the colonized patient group did not differ significantly from those in the Pneumocystis-uninfected patients group (P=. 0.21). Conclusion: The results suggest that measurements of BAL BG levels may facilitate the differential diagnosis of PCP and pulmonary colonization with Pneumocystis.

KW - (1,3) ß-d-glucan

KW - Bronchoalveolar lavage samples

KW - Pneumocystis pneumonia

KW - Pneumocystis pulmonary colonization

UR - https://www.scopus.com/pages/publications/84924444936

U2 - 10.1016/j.mycmed.2014.11.001

DO - 10.1016/j.mycmed.2014.11.001

M3 - Article

C2 - 25498852

AN - SCOPUS:84924444936

SN - 1156-5233

VL - 25

SP - 36

EP - 43

JO - Journal de Mycologie Medicale

JF - Journal de Mycologie Medicale

IS - 1

ER -

Usefulness of (1,3) ß-d-glucan detection in bronchoalveolar lavage samples in Pneumocystis pneumonia and Pneumocystis pulmonary colonization

Abstract

UN SDGs

Keywords

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