Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.)

L. Hartl, H. Weiss, F. J. Zeller, A. Jahoor

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73 Scopus citations

Abstract

The objective of this study was to identify molecular markers linked to genes for resistance to powdery mildew (Pm) in wheat using a series of 'Chancellor' near-isogenic-lines (NILs), each having one powdery mildew resistance gene. A total of 210 probes were screened for their ability to detect polymorphism between the NILs and the recurrent parent. One of these restriction fragment length polymorphism (RFLP) markers (Xwhs179) revealed polymorphism not only between the NILs for the Pm3 locus, but also among NILs possessing different alleles of the Pm3 locus. The location of the marker Xwhs179 was confirmed to be on homoeologous chromosome group 1 with the help of nullitetrasomic wheat lines. The linkage relationship between this probe and the Pm3 locus was estimated with double haploid lines derived from a cross between wheat cvs 'Club' and 'Chul' (Pm3b). The genetic distance was determined to be 3.3±1.9 cM.

Original languageEnglish
Pages (from-to)959-963
Number of pages5
JournalTheoretical and Applied Genetics
Volume86
Issue number8
DOIs
StatePublished - Sep 1993

Keywords

  • Mildew resistance
  • Near-isogenic lines
  • Pm3 locus
  • RFLP marker
  • Triticum aestivum

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