Use of double-replacement gene targeting to replace the murine α-lactalbumin gene with its human counterpart in embryonic stem cells and mice

Alexander Stacey, Angelika Schnieke, Jim McWhir, Julian Cooper, Alan Colman, David W. Melton

Research output: Contribution to journalArticlepeer-review

111 Scopus citations

Abstract

The mouse α-lactalbumin gene has been replaced with the human gene by two consecutive rounds of gene targeting in hypoxanthine phosphoribosyltransferase (HPRT)-deficient feeder-independent murine embryonic stem (ES) cells. One mouse α-lactalbumin allele was first replaced by an HPRT minigene which was in turn replaced by human α-lactalbumin. The end result is a clean exchange of defined DNA fragments with no other DNA remaining at the target locus. Targeted ES cells at each stage remained capable of contributing efficiently to the germ line of chimeric animals. Double replacement using HPRT-deficient ES cells and the HPRT selection system is therefore a powerful and flexible method of targeting specific alterations to animal genes. A typical strategy for future use would be to generate a null mutation which could then be used to produce multiple second-step alterations at the same locus.

Original languageEnglish
Pages (from-to)1009-1016
Number of pages8
JournalMolecular and Cellular Biology
Volume14
Issue number2
StatePublished - Feb 1994
Externally publishedYes

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