Abstract
Polyclonal antibodies were prepared against the purified elongation factor Tu (EF-Tu) of Escherichia coli and Bacillus subtilis. Using the methods of Western blotting and microcomplement fixation the cross-reactivities of EF-Tu of 19 different prokaryotes were determined. The immunological distance were compared with the results of 16S rRNA oligonucleotide analysis. An unexpectedly high cross-reactivity was revealed between the EF-Tu of B. subtilis and the antiserum against the EF-Tu of E. coli. A comparison of the predicted amino acid sequences from the tuf-genes of E. coli and B. subtilis yielded two identical peptide fragments that are likely candidates for antibody binding sites.
| Original language | English |
|---|---|
| Pages (from-to) | 258-262 |
| Number of pages | 5 |
| Journal | Archives of Microbiology |
| Volume | 152 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 1989 |
Keywords
- Antibody binding site
- Cross-reactivity
- Elongation factor Tu
- Microcomplement fixation
- Western blot
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