TY - JOUR
T1 - Two‐dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimensin (IPG‐Dalt)
T2 - The state of the art and the controversy of vertical versus horizontal systems
AU - Görg, Angelika
AU - Boguth, GÜNther
AU - Obermaier, Christian
AU - Posch, Anton
AU - Weiss, Walter
PY - 1995
Y1 - 1995
N2 - After having established the basic protocol of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension (IPG‐Dalt) in 1988 (A. Görg et al., Electrophoresis 1988, 9, 531–546), some critical parameters of the actual IPG‐Dalt protocols as well as the results obtained with horizontal and vertical second‐dimensional sodium dodecyl sulfate‐electrophoresis are demonstrated and discussed.
AB - After having established the basic protocol of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension (IPG‐Dalt) in 1988 (A. Görg et al., Electrophoresis 1988, 9, 531–546), some critical parameters of the actual IPG‐Dalt protocols as well as the results obtained with horizontal and vertical second‐dimensional sodium dodecyl sulfate‐electrophoresis are demonstrated and discussed.
KW - Immobilized pH gradients
KW - Two‐dimensional polyacrylamide gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0029095434&partnerID=8YFLogxK
U2 - 10.1002/elps.11501601183
DO - 10.1002/elps.11501601183
M3 - Article
C2 - 7498150
AN - SCOPUS:0029095434
SN - 0173-0835
VL - 16
SP - 1079
EP - 1086
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 1
ER -