TWO-PHOTON MICROSCOPY AND ACOUSTIC TRAPPING FOR THE ANALYSIS OF CELL OSMOSIS

Bettina Sailer, Thomas Kellerer, Rune Barnkob, Thomas Hellerer, Oliver Hayden

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Acoustic trapping of red blood cells (RBCs) in a spherical microchamber (SMC) are demonstrated for the manipulation and analysis of cellular microaggregates under microscopy methods brightfield and two-photon excited fluorescence (TPEF). We envision a platform technology for non-invasive accumulating of cells in 3D without a wall contact in microfluidic flow conditions and compare both microscopy methods for optical analysis of cell functions and cell interactions using osmosis in RBCs as example. Future work on our system combined with TPEF will be directed towards cell function diagnostics covering a diagnostic gap of in-vitro cell testing in 3D for biomedical research.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages897-898
Number of pages2
ISBN (Electronic)9781733419031
StatePublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 10 Oct 202114 Oct 2021

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period10/10/2114/10/21

Keywords

  • Acoustofluidics
  • Cell Trapping
  • Osmosis
  • Spherical Microchamber
  • Two-Photon-Microscopy

Fingerprint

Dive into the research topics of 'TWO-PHOTON MICROSCOPY AND ACOUSTIC TRAPPING FOR THE ANALYSIS OF CELL OSMOSIS'. Together they form a unique fingerprint.

Cite this