TY - JOUR
T1 - Trehalose production by Cupriavidus necator from CO2 and hydrogen gas
AU - Löwe, Hannes
AU - Beentjes, Marleen
AU - Pflüger-Grau, Katharina
AU - Kremling, Andreas
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2021/1
Y1 - 2021/1
N2 - In this work, the hydrogen-oxidizing bacterium Cupriavidus necator H16 was engineered for trehalose production from gaseous substrates. First, it could be shown that C. necator is a natural producer of trehalose when stressed with sodium chloride. Bioinformatic investigations revealed a so far unknown mode of trehalose and glycogen metabolism in this organism. Next, it was found that expression of the sugar efflux transporter A (setA) from Escherichia coli lead to a trehalose leaky phenotype of C. necator. Finally, the strain was characterized under autotrophic conditions using a H2/CO2/O2-mixture and other substrates reaching titers of up to 0.47 g L−1 and yields of around 0.1 g g−1. Taken together, this process represents a new way to produce sugars with high areal efficiency. With further metabolic engineering, an application of this technology for the renewable production of trehalose and other sugars, as well as for the synthesis of 13C-labeled sugars seems promising.
AB - In this work, the hydrogen-oxidizing bacterium Cupriavidus necator H16 was engineered for trehalose production from gaseous substrates. First, it could be shown that C. necator is a natural producer of trehalose when stressed with sodium chloride. Bioinformatic investigations revealed a so far unknown mode of trehalose and glycogen metabolism in this organism. Next, it was found that expression of the sugar efflux transporter A (setA) from Escherichia coli lead to a trehalose leaky phenotype of C. necator. Finally, the strain was characterized under autotrophic conditions using a H2/CO2/O2-mixture and other substrates reaching titers of up to 0.47 g L−1 and yields of around 0.1 g g−1. Taken together, this process represents a new way to produce sugars with high areal efficiency. With further metabolic engineering, an application of this technology for the renewable production of trehalose and other sugars, as well as for the synthesis of 13C-labeled sugars seems promising.
KW - Bioeconomy
KW - C-labelled sugar
KW - Cupriavidus necator
KW - Hydrogen-oxidizing bacteria
KW - Sugar efflux transporter
KW - Syngas fermentation
KW - Trehalose
UR - http://www.scopus.com/inward/record.url?scp=85092531512&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2020.124169
DO - 10.1016/j.biortech.2020.124169
M3 - Article
C2 - 33254445
AN - SCOPUS:85092531512
SN - 0960-8524
VL - 319
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 124169
ER -