Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype

Tilo Kunath, Gerald Gish, Heiko Lickert, Nina Jones, Tony Pawson, Janet Rossant

Research output: Contribution to journalArticlepeer-review

254 Scopus citations

Abstract

Gene targeting via homologous recombination in murine embryonic stem (ES) cells has been the method of choice for deciphering mammalian gene function in vivo. Despite improvements in this technology, it still remains a laborious method. Recent advances in RNA interference (RNAi) technology have provided a rapid loss-of-function method for assessing gene function in a number of organisms. Studies in mammalian cell lines have shown that introduction of small interfering RNA (siRNA) molecules mediates effective RNA silencing. Plasmid-based systems using RNA polymerase III (RNA pol III) promoters to drive short hairpin RNA (shRNA) molecules were established to stably produce siRNA. Here we report the generation of knockdown ES cell lines with transgenic shRNA. Because of the dominant nature of the knockdown, embryonic phenotypes could be directly assessed in embryos completely derived from ES cells by the tetraploid aggregation method. Such embryos, in which endogenous p120-Ras GTPase-activating protein (RasGAP), encoded by Rasa1 (also known as RasGAP), was silenced, had the same phenotype as did the previously reported Rasa1 null mutation.

Original languageEnglish
Pages (from-to)559-561
Number of pages3
JournalNature Biotechnology
Volume21
Issue number5
DOIs
StatePublished - 1 May 2003
Externally publishedYes

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