Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue

Julius Honecker; Stefan Ruschke; Claudine Seeliger; Samantha Laber; Sophie Strobel; Priska Pröll; Christoffer Nellaker; Cecilia M. Lindgren; Ulrich Kulozik; Josef Ecker; Dimitrios C. Karampinos; Melina Claussnitzer; Hans Hauner

doi:10.1016/j.ebiom.2022.104020

Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue

Julius Honecker
, Stefan Ruschke
, Claudine Seeliger
, Samantha Laber
, Sophie Strobel
, Priska Pröll
, Christoffer Nellaker
, Cecilia M. Lindgren
, Ulrich Kulozik
, Josef Ecker
, Dimitrios C. Karampinos
, Melina Claussnitzer
, Hans Hauner

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Background: The adipocyte-hypertrophy associated remodeling of fat cell function is considered causal for the development of metabolic disorders. A better understanding of transcriptome and fatty acid (FA) related alterations with adipocyte hypertrophy combined with less-invasive strategies for the detection of the latter can help to increase the prognostic and diagnostic value of adipocyte size and FA composition as markers for metabolic disease. Methods: To clarify adipocyte-hypertrophy associated transcriptomic alterations, fat cell size was related to RNA-Seq data from white adipose tissue and size-separated adipocytes. The relationship between adipocyte size and adipose tissue FA composition as measured by GC-MS was investigated. MR spectroscopy (MRS) methods for clinical scanning were developed to characterize adipocyte size and FA composition in a fast and non-invasive manner. Findings: With enlarged adipocyte size, substantial transcriptomic alterations of genes involved in mitochondrial function and FA metabolism were observed. Investigations of these two mechanisms revealed a reciprocal relationship between adipocyte size and estimated thermogenic adipocyte content as well as depot-specific correlations of adipocyte size and FA composition. MRS on a clinical scanner was suitable for the in-parallel assessment of adipose morphology and FA composition. Interpretation: The current study provides a comprehensive overview of the adipocyte-hypertrophy associated transcriptomic and FA landscape in both subcutaneous and visceral adipose tissue. MRS represents a promising technique to translate the observed mechanistic, structural and functional changes in WAT with adipocyte hypertrophy into a clinical context for an improved phenotyping of WAT in the context of metabolic diseases. Funding: Competence network for obesity (FKZ 42201GI1128), ERC (No 677661, ProFatMRI; No 875488, FatVirtualBiopsy), Else Kröner-Fresenius-Foundation.

Original language	English
Article number	104020
Journal	eBioMedicine
Volume	79
DOIs	https://doi.org/10.1016/j.ebiom.2022.104020
State	Published - May 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Browning
Fatty acids
Magnetic resonance
Obesity
Transcriptomics
White adipose tissue

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10.1016/j.ebiom.2022.104020

Cite this

Honecker, J., Ruschke, S., Seeliger, C., Laber, S., Strobel, S., Pröll, P., Nellaker, C., Lindgren, C. M., Kulozik, U., Ecker, J., Karampinos, D. C., Claussnitzer, M., & Hauner, H. (2022). Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue. eBioMedicine, 79, Article 104020. https://doi.org/10.1016/j.ebiom.2022.104020

@article{72bd70b4eeb44f2db945ab0c48212397,

title = "Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue",

abstract = "Background: The adipocyte-hypertrophy associated remodeling of fat cell function is considered causal for the development of metabolic disorders. A better understanding of transcriptome and fatty acid (FA) related alterations with adipocyte hypertrophy combined with less-invasive strategies for the detection of the latter can help to increase the prognostic and diagnostic value of adipocyte size and FA composition as markers for metabolic disease. Methods: To clarify adipocyte-hypertrophy associated transcriptomic alterations, fat cell size was related to RNA-Seq data from white adipose tissue and size-separated adipocytes. The relationship between adipocyte size and adipose tissue FA composition as measured by GC-MS was investigated. MR spectroscopy (MRS) methods for clinical scanning were developed to characterize adipocyte size and FA composition in a fast and non-invasive manner. Findings: With enlarged adipocyte size, substantial transcriptomic alterations of genes involved in mitochondrial function and FA metabolism were observed. Investigations of these two mechanisms revealed a reciprocal relationship between adipocyte size and estimated thermogenic adipocyte content as well as depot-specific correlations of adipocyte size and FA composition. MRS on a clinical scanner was suitable for the in-parallel assessment of adipose morphology and FA composition. Interpretation: The current study provides a comprehensive overview of the adipocyte-hypertrophy associated transcriptomic and FA landscape in both subcutaneous and visceral adipose tissue. MRS represents a promising technique to translate the observed mechanistic, structural and functional changes in WAT with adipocyte hypertrophy into a clinical context for an improved phenotyping of WAT in the context of metabolic diseases. Funding: Competence network for obesity (FKZ 42201GI1128), ERC (No 677661, ProFatMRI; No 875488, FatVirtualBiopsy), Else Kr{\"o}ner-Fresenius-Foundation.",

keywords = "Browning, Fatty acids, Magnetic resonance, Obesity, Transcriptomics, White adipose tissue",

author = "Julius Honecker and Stefan Ruschke and Claudine Seeliger and Samantha Laber and Sophie Strobel and Priska Pr{\"o}ll and Christoffer Nellaker and Lindgren, \{Cecilia M.\} and Ulrich Kulozik and Josef Ecker and Karampinos, \{Dimitrios C.\} and Melina Claussnitzer and Hans Hauner",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = may,

doi = "10.1016/j.ebiom.2022.104020",

language = "English",

volume = "79",

journal = "eBioMedicine",

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Honecker, J, Ruschke, S, Seeliger, C, Laber, S, Strobel, S, Pröll, P, Nellaker, C, Lindgren, CM, Kulozik, U, Ecker, J, Karampinos, DC, Claussnitzer, M & Hauner, H 2022, 'Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue', eBioMedicine, vol. 79, 104020. https://doi.org/10.1016/j.ebiom.2022.104020

TY - JOUR

T1 - Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue

AU - Honecker, Julius

AU - Ruschke, Stefan

AU - Seeliger, Claudine

AU - Laber, Samantha

AU - Strobel, Sophie

AU - Pröll, Priska

AU - Nellaker, Christoffer

AU - Lindgren, Cecilia M.

AU - Kulozik, Ulrich

AU - Ecker, Josef

AU - Karampinos, Dimitrios C.

AU - Claussnitzer, Melina

AU - Hauner, Hans

PY - 2022/5

Y1 - 2022/5

N2 - Background: The adipocyte-hypertrophy associated remodeling of fat cell function is considered causal for the development of metabolic disorders. A better understanding of transcriptome and fatty acid (FA) related alterations with adipocyte hypertrophy combined with less-invasive strategies for the detection of the latter can help to increase the prognostic and diagnostic value of adipocyte size and FA composition as markers for metabolic disease. Methods: To clarify adipocyte-hypertrophy associated transcriptomic alterations, fat cell size was related to RNA-Seq data from white adipose tissue and size-separated adipocytes. The relationship between adipocyte size and adipose tissue FA composition as measured by GC-MS was investigated. MR spectroscopy (MRS) methods for clinical scanning were developed to characterize adipocyte size and FA composition in a fast and non-invasive manner. Findings: With enlarged adipocyte size, substantial transcriptomic alterations of genes involved in mitochondrial function and FA metabolism were observed. Investigations of these two mechanisms revealed a reciprocal relationship between adipocyte size and estimated thermogenic adipocyte content as well as depot-specific correlations of adipocyte size and FA composition. MRS on a clinical scanner was suitable for the in-parallel assessment of adipose morphology and FA composition. Interpretation: The current study provides a comprehensive overview of the adipocyte-hypertrophy associated transcriptomic and FA landscape in both subcutaneous and visceral adipose tissue. MRS represents a promising technique to translate the observed mechanistic, structural and functional changes in WAT with adipocyte hypertrophy into a clinical context for an improved phenotyping of WAT in the context of metabolic diseases. Funding: Competence network for obesity (FKZ 42201GI1128), ERC (No 677661, ProFatMRI; No 875488, FatVirtualBiopsy), Else Kröner-Fresenius-Foundation.

AB - Background: The adipocyte-hypertrophy associated remodeling of fat cell function is considered causal for the development of metabolic disorders. A better understanding of transcriptome and fatty acid (FA) related alterations with adipocyte hypertrophy combined with less-invasive strategies for the detection of the latter can help to increase the prognostic and diagnostic value of adipocyte size and FA composition as markers for metabolic disease. Methods: To clarify adipocyte-hypertrophy associated transcriptomic alterations, fat cell size was related to RNA-Seq data from white adipose tissue and size-separated adipocytes. The relationship between adipocyte size and adipose tissue FA composition as measured by GC-MS was investigated. MR spectroscopy (MRS) methods for clinical scanning were developed to characterize adipocyte size and FA composition in a fast and non-invasive manner. Findings: With enlarged adipocyte size, substantial transcriptomic alterations of genes involved in mitochondrial function and FA metabolism were observed. Investigations of these two mechanisms revealed a reciprocal relationship between adipocyte size and estimated thermogenic adipocyte content as well as depot-specific correlations of adipocyte size and FA composition. MRS on a clinical scanner was suitable for the in-parallel assessment of adipose morphology and FA composition. Interpretation: The current study provides a comprehensive overview of the adipocyte-hypertrophy associated transcriptomic and FA landscape in both subcutaneous and visceral adipose tissue. MRS represents a promising technique to translate the observed mechanistic, structural and functional changes in WAT with adipocyte hypertrophy into a clinical context for an improved phenotyping of WAT in the context of metabolic diseases. Funding: Competence network for obesity (FKZ 42201GI1128), ERC (No 677661, ProFatMRI; No 875488, FatVirtualBiopsy), Else Kröner-Fresenius-Foundation.

KW - Browning

KW - Fatty acids

KW - Magnetic resonance

KW - Obesity

KW - Transcriptomics

KW - White adipose tissue

UR - https://www.scopus.com/pages/publications/85129566762

U2 - 10.1016/j.ebiom.2022.104020

DO - 10.1016/j.ebiom.2022.104020

M3 - Article

C2 - 35490555

AN - SCOPUS:85129566762

SN - 2352-3964

VL - 79

JO - eBioMedicine

JF - eBioMedicine

M1 - 104020

ER -

Transcriptome and fatty-acid signatures of adipocyte hypertrophy and its non-invasive MR-based characterization in human adipose tissue

Abstract

UN SDGs

Keywords

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