Transcriptome analysis in vulvar squamous cell cancer

Katharina Prieske, Malik Alawi, Anna Jaeger, Maximilian Christian Wankner, Kathrin Eylmann, Susanne Reuter, Patrick Lebok, Eike Burandt, Niclas C. Blessin, Barbara Schmalfeldt, Leticia Oliveira-Ferrer, Simon A. Joosse, Linn Woelber

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

To date, therapeutic strategies in vulvar squamous cell carcinoma (VSCC) are lacking molecular pathological information and targeted therapy hasn’t been approved in the treatment of VSCC, yet. Two etiological pathways are widely accepted: HPV induced vs. HPV independent, associated with chronic skin disease, often harboring TP53 mutations (mut). The aim of this analysis was to analyze the RNA expression patterns for subtype stratification on VSCC samples that can be integrated into the previously performed whole exome sequencing data for the detection of prognostic markers and potential therapeutic targets. We performed multiplex gene expression analysis (NanoString) with 770 genes in 24 prior next generation sequenced samples. An integrative data analysis was performed. Here, 98 genes were differentially expressed in TP53mut vs. HPV+ VSCC, in the TP53mut cohort, where 56 genes were upregulated and 42 were downregulated in comparison to the HPV+ tumors. Aberrant expression was primarily observed in cell cycle regulation, especially in HPV+ disease. Within the TP53mut group, a distinct cluster was identified that was correlated to a significantly worse overall survival (p = 0.017). The RNA expression profiles showed distinct patterns with regard to the known VSCC subtypes and could potentially enable further subclassification in the TP53mut groups.

Original languageEnglish
Article number6372
JournalCancers
Volume13
Issue number24
DOIs
StatePublished - 1 Dec 2021
Externally publishedYes

Keywords

  • HPV 5
  • RNA 6
  • Sequencing 2
  • TP53 4
  • Transcriptome 3
  • Vulvar cancer 1

Fingerprint

Dive into the research topics of 'Transcriptome analysis in vulvar squamous cell cancer'. Together they form a unique fingerprint.

Cite this