Transcriptional response reveals translation machinery as target for high pressure in Lactobacillus sanfranciscensis

Melanie Pavlovic, Sebastian Hörmann, Rudi F. Vogel, Matthias A. Ehrmann

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The effect of sublethal hydrostatic pressure on the transcriptome of Lactobacillus sanfranciscensis was determined using a shot-gun-microarray. Among the 750 spots that passed quality analysis 42 genes were induced, while six were repressed when cells were incubated at 45 MPa for 30 min. The nature of genes and their differential expression clearly indicate cellular efforts to counteract a decrease in translational capacity. The majority of high pressure affected genes were found to encode either translation factors (EF-G, EF-TU), ribosomal proteins (S2, L6, L11), genes changing translational accuracy or molecular chaperones (GroEL, ClpL). These data agree with previously reported effects observed in in vitro studies as well as with physiological and proteomic data. This study provides in vivo evidence to identify ribosomes and impaired translation among primary targets for high pressure treatment. The observed induction of heat as well as cold shock genes (e.g. hsp60, gyrA) may be explained as a result of high pressure affected protein synthesis.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalArchives of Microbiology
Volume184
Issue number1
DOIs
StatePublished - Oct 2005

Keywords

  • High hydrostatic pressure
  • Lactic acid bacteria
  • Lactobacillus sanfranciscensis
  • Stress response
  • Transcriptome

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