Transcriptional regulation of α5β1 integrin, fibronectin, VCAM-1, MCSF-1/c-fms, and MCP-1 in atrioventricular valves after valvular surgery and Staphylococcus aureus bacteremia

Walter Benno Eichinger, Joachim Burkhard Grammer, Bo Zhao, Juliane Brückner, Nikolaus Mendler, Rüdiger Lange, Robert Bauernschmitt

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Objective: The primary event in the development of infective endocarditis (IE) is bacterial adherence to damaged heart valves. This includes capture, adhesion and internalization of bacteria into host cells. Methods: We determined in an experimental rabbit model for IE whether a transient bacteremia caused by Staphylococcus aureus and/or endothelial heart valve lesions induce transcriptional regulation of α5β1 integrin, fibronectin, vascular cell adhesion molecule-1 (VCAM-1), macrophage colony-stimulating factor-1 (MCSF-1), c-fms proto-oncogene (MCSF-1 receptor), and macrophage chemoattractant protein-1 (MCP-1) in mitral and tricuspid valves. Results: No significant upregulation was found after isolated bacteremia. Six hours after surgical manipulation valvular endothelial lesions led to a distinct modulation in the mRNA expression of proinflammatory cytokines (MCSF-1 and MCP-1), VCAM-1 and α5β1 integrin. The most evident differences between the mitral and tricuspid valves were seen in the significant upregulation of VCAM-1 mRNA on the tricuspid valve in the surgical groups, whereas there was no effect on the mitral valve. MCSF-1 and MCP-1 were dramatically upregulated in both valves after surgery. Conclusions: During the host defence mechanisms in the development of IE proinflammatory cytokines, cellular adhesion molecules, and molecules of the fibronectin/integrin axis are activated, showing distinct differences in right- and left-sided heart valves.

Original languageEnglish
Pages (from-to)465-472
Number of pages8
JournalCytokine
Volume31
Issue number6
DOIs
StatePublished - 21 Sep 2005

Keywords

  • Adhesion molecules
  • Cytokines
  • Endocarditis
  • Gene expression
  • Heart valves

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