TY - JOUR
T1 - Toxicity and genetic diversity of indoor and outdoor stachybotrys chartarum of different geographic origins
AU - Peltola, Joanna
AU - Niessen, Ludwig
AU - Nielsen, Kristian F.
AU - Jarvis, Bruce B.
AU - Salkinoja-Salonen, Mirja
AU - Möller, Evelyn M.
PY - 2001
Y1 - 2001
N2 - Thirty-one isolates of the genus Stachybotrys from indoor and outdoor environments were analyzed for presence of trichodiene synthase gene (Tri5), production of trichothecenes, boar sperm cell motility inhibition, and randomly amplified polymorphic DNA banding patterns (RAPDs). Stachybotrys chartarum isolates (n = 22) possessing the Tri5 gene (encodes the trichodiene synthase enzyme needed at the early steps of trichothecene synthesis) had similar RAPD fingerprints to each other, and 16 of these isolates contained satratoxins or trichodermol. Nine S. chartarum isolates, which were negative for Tri5 gene and trichothecenes, were less than 50% similar to the Tri5 positive group in RAPD fingerprints, but similar to each other. Nineteen of the isolates inhibited sperm motility at the level of EC50 < 60 μg of crude extract mL-1. Toxicity observed by inhibition of the boar spermatozoan motility was distributed among the two RAPD groups of S. chartarum.
AB - Thirty-one isolates of the genus Stachybotrys from indoor and outdoor environments were analyzed for presence of trichodiene synthase gene (Tri5), production of trichothecenes, boar sperm cell motility inhibition, and randomly amplified polymorphic DNA banding patterns (RAPDs). Stachybotrys chartarum isolates (n = 22) possessing the Tri5 gene (encodes the trichodiene synthase enzyme needed at the early steps of trichothecene synthesis) had similar RAPD fingerprints to each other, and 16 of these isolates contained satratoxins or trichodermol. Nine S. chartarum isolates, which were negative for Tri5 gene and trichothecenes, were less than 50% similar to the Tri5 positive group in RAPD fingerprints, but similar to each other. Nineteen of the isolates inhibited sperm motility at the level of EC50 < 60 μg of crude extract mL-1. Toxicity observed by inhibition of the boar spermatozoan motility was distributed among the two RAPD groups of S. chartarum.
UR - http://www.scopus.com/inward/record.url?scp=84874142034&partnerID=8YFLogxK
M3 - Conference article
AN - SCOPUS:84874142034
SN - 2166-4870
JO - IAQ Conference
JF - IAQ Conference
T2 - Moisture, Microbes and Health Effects: Indoor Air Quality and Moisture in Buildings Conference, IAQ 2001
Y2 - 4 November 2001 through 7 November 2001
ER -
