Towards a Point-of-Care Test of CD4+ T Lymphocyte Concentrations for Immune Status Monitoring with Magnetic Flow Cytometry

Moritz Leuthner, Mathias Reisbeck, Michael Helou, Oliver Hayden

Research output: Contribution to journalArticlepeer-review

Abstract

For the treatment of human immunodeficiency virus (HIV)-infected patients, the regular assessment of the immune status is indispensable. The quantification of CD4+ T lymphocytes in blood by gold standard optical flow cytometry is not point-of-care testing (POCT) compatible. This incompatibility is due to unavoidable pre-analytics, expensive and bulky optics with limited portability, and complex workflow integration. Here, we propose a non-optical, magnetic flow cytometry (MFC) workflow that offers effortless integration opportunities, including minimal user interaction, integrated sample preparation and up-concentration, and miniaturization. Furthermore, we demonstrate immunomagnetic CD4+ T lymphocyte labeling in whole blood with subsequent quantification using sheath-less MFC. Showing linearity over two log scales and being largely unimpaired by hematocrit, evidence is provided for POCT capabilities of HIV patients.

Original languageEnglish
Article number520
JournalMicromachines
Volume15
Issue number4
DOIs
StatePublished - Apr 2024

Keywords

  • flow cytometry
  • human immunodeficiency virus (HIV)
  • immune status
  • lab-on-a-chip (LOC)
  • magnetic flow cytometry
  • point-of-care testing (POCT)
  • whole blood

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