Toward one-pot lipase-catalyzed synthesis of poly(e-caprolactone) particles in aqueous dispersion

Pranee Inprakhon, Pabhangkon Panlawan, Thunyarat Pongtharankul, Emmanuelle Marie, Lars O. Wiemann, Alain Durand, Volker Sieber

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The preparation of polyester particles using enzyme-catalyzed (lipase from Candida antarctica B, CALB) ring-opening polymerization of e-caprolactone (e-CL) in aqueous dispersion was demonstrated for the first time. Immobilization of CALB enabled a significant increase of the number-average degree of polymerization of e-CL oligomers (up to 38) as compared to dissolved CALB (8 at the maximum). The nature and amount of lipase, as well as the nature of the support material were identified as key parameters controlling ring-opening polymerization of e-CL in aqueous dispersion. In addition, the involvement of solubilized monomers in polymerization elementary reactions was demonstrated and the consequences on oligomers average length were detailed. An overall mechanism of lipase-catalyzed e-CL polymerization in aqueous dispersion taking into account the colloidal nature of reaction medium was proposed on the basis of experimental results.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume113
DOIs
StatePublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Aqueous dispersion
  • Lipase
  • Ring-opening polymerization
  • e-caprolactone

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