TY - JOUR
T1 - Total biliary protein, mucus glycoproteins, cyclic-AMP, and apolipoproteins in the gallbladder bile of patients with cholesterol stones and Stone-free controls
AU - Swobodnik, W.
AU - Wenk, H.
AU - Janowitz, P.
AU - Hagert, N.
AU - Kratzer, W.
AU - Berghold, J.
AU - Zhang, Y.
AU - Bittner, R.
AU - Schusdziarra, V.
AU - Ott, R.
AU - Kuhn, K.
AU - Classen, M.
PY - 1991
Y1 - 1991
N2 - The concentrations of total protein, mucus glycoprotein, cyclic-AMP, and apolipoproteins A-I, A-II, and B were determined in the gallbladder bile of patients with cholesterol gallbladder stones and in stone-free controls. The total protein content was significantly increased in gallstone patients (2.03 ± 0.6 versus 1.31 ± 0.67 mg/ ml; p < 0.05), as was the mucus glycoprotein concentration (380 ± 88.5 versus 128 ± 57.2 ug/ml; p < 0.05). The cyclic-AMP concentration in the gallbladder fluid was increased up to 91 ± 20 pmol/100 ul in the gallstone subjects, as compared with 46 ± 26 pmol/100 ul (p < 0.01) in stone-free controls. Cyclic-AMP concentrations correlated positively with the glycoprotein content of the bile in cholesterol gallstone patients (r = 0.66; p <0.05). The apolipoprotein concentrations were determined by the radial immundiffusion technique. The corresponding values for patients with stones and controls were 7.5 ± 0.8 verus 3.0 + 0.8 for Apo A-I (p < 0.025), 10.4 ±0.6 versus 6.3 ± 1.3 for Apo A-II (p < 0.02), and 1.9 ± 0.5 versus 1.6 ± 0.2 mg/dl for Apo B (NS), respectively. Biliary proteins probably play an important role in the nucleation process during the pathogenesis of cholesterol gallbladder stones.
AB - The concentrations of total protein, mucus glycoprotein, cyclic-AMP, and apolipoproteins A-I, A-II, and B were determined in the gallbladder bile of patients with cholesterol gallbladder stones and in stone-free controls. The total protein content was significantly increased in gallstone patients (2.03 ± 0.6 versus 1.31 ± 0.67 mg/ ml; p < 0.05), as was the mucus glycoprotein concentration (380 ± 88.5 versus 128 ± 57.2 ug/ml; p < 0.05). The cyclic-AMP concentration in the gallbladder fluid was increased up to 91 ± 20 pmol/100 ul in the gallstone subjects, as compared with 46 ± 26 pmol/100 ul (p < 0.01) in stone-free controls. Cyclic-AMP concentrations correlated positively with the glycoprotein content of the bile in cholesterol gallstone patients (r = 0.66; p <0.05). The apolipoprotein concentrations were determined by the radial immundiffusion technique. The corresponding values for patients with stones and controls were 7.5 ± 0.8 verus 3.0 + 0.8 for Apo A-I (p < 0.025), 10.4 ±0.6 versus 6.3 ± 1.3 for Apo A-II (p < 0.02), and 1.9 ± 0.5 versus 1.6 ± 0.2 mg/dl for Apo B (NS), respectively. Biliary proteins probably play an important role in the nucleation process during the pathogenesis of cholesterol gallbladder stones.
KW - Apolipoprotein
KW - Biliary protein
KW - Cyclic-AMP
KW - Gallstone pathogenesis
KW - Glycoprotein
UR - http://www.scopus.com/inward/record.url?scp=0025826977&partnerID=8YFLogxK
U2 - 10.3109/00365529108998598
DO - 10.3109/00365529108998598
M3 - Article
C2 - 1654593
AN - SCOPUS:0025826977
SN - 0036-5521
VL - 26
SP - 771
EP - 778
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
IS - 7
ER -