TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses

Eva Schlosser; Marc Mueller; Stefan Fischer; Sameh Basta; Dirk H. Busch; Bruno Gander; Marcus Groettrup

doi:10.1016/j.vaccine.2008.01.030

TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses

Eva Schlosser
, Marc Mueller
, Stefan Fischer
, Sameh Basta
, Dirk H. Busch
, Bruno Gander
, Marcus Groettrup

Chair of Medical Microbiology, Immunology and Hygiene

Division of Immunology
ETH Zurich
Queen's University
Technical University of Munich
Biotechnology Institute Thurgau

Research output: Contribution to journal › Article › peer-review

243 Scopus citations

Abstract

Dendritic cells phagocytose pathogens leading to maturation and cross-presentation on MHC class I. We found that the efficiency of cross-priming in mice after vaccination with biodegradable poly(d,l-lactide-co-glycolide) microspheres (MSs) was enhanced when ovalbumin was coencapsulated together with either a CpG oligonucleotide or polyI:C as compared to co-inoculation of ovalbumin-bearing MS with soluble or separately encapsulated adjuvants. A single immunization with MS containing coencaspsulated CpG and ovalbumin yielded 9% SIINFEKL/H-2K^b tetramer positive CTLs, production of IFN-γ, efficient cytolysis, and protection from vaccinia virus infection. Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses.

Original language	English
Pages (from-to)	1626-1637
Number of pages	12
Journal	Vaccine
Volume	26
Issue number	13
DOIs	https://doi.org/10.1016/j.vaccine.2008.01.030
State	Published - 20 Mar 2008

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biodegradable microspheres
Cytotoxic T cells
Viral infection

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10.1016/j.vaccine.2008.01.030

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title = "TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses",

abstract = "Dendritic cells phagocytose pathogens leading to maturation and cross-presentation on MHC class I. We found that the efficiency of cross-priming in mice after vaccination with biodegradable poly(d,l-lactide-co-glycolide) microspheres (MSs) was enhanced when ovalbumin was coencapsulated together with either a CpG oligonucleotide or polyI:C as compared to co-inoculation of ovalbumin-bearing MS with soluble or separately encapsulated adjuvants. A single immunization with MS containing coencaspsulated CpG and ovalbumin yielded 9\% SIINFEKL/H-2Kb tetramer positive CTLs, production of IFN-γ, efficient cytolysis, and protection from vaccinia virus infection. Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses.",

keywords = "Biodegradable microspheres, Cytotoxic T cells, Viral infection",

author = "Eva Schlosser and Marc Mueller and Stefan Fischer and Sameh Basta and Busch, \{Dirk H.\} and Bruno Gander and Marcus Groettrup",

note = "Funding Information: We thank Antje Heit and Hermann Wagner for scientific advice and stimulating discussions, Ying Waeckerle-Men for initial help with MS production, and Michael Basler for sharing his experience with CTL assays. This work was supported by grant no. 10-2156 (MG) of Deutsche Krebshilfe e.V.",

year = "2008",

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day = "20",

doi = "10.1016/j.vaccine.2008.01.030",

language = "English",

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TY - JOUR

T1 - TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses

AU - Schlosser, Eva

AU - Mueller, Marc

AU - Fischer, Stefan

AU - Basta, Sameh

AU - Busch, Dirk H.

AU - Gander, Bruno

AU - Groettrup, Marcus

N1 - Funding Information: We thank Antje Heit and Hermann Wagner for scientific advice and stimulating discussions, Ying Waeckerle-Men for initial help with MS production, and Michael Basler for sharing his experience with CTL assays. This work was supported by grant no. 10-2156 (MG) of Deutsche Krebshilfe e.V.

PY - 2008/3/20

Y1 - 2008/3/20

N2 - Dendritic cells phagocytose pathogens leading to maturation and cross-presentation on MHC class I. We found that the efficiency of cross-priming in mice after vaccination with biodegradable poly(d,l-lactide-co-glycolide) microspheres (MSs) was enhanced when ovalbumin was coencapsulated together with either a CpG oligonucleotide or polyI:C as compared to co-inoculation of ovalbumin-bearing MS with soluble or separately encapsulated adjuvants. A single immunization with MS containing coencaspsulated CpG and ovalbumin yielded 9% SIINFEKL/H-2Kb tetramer positive CTLs, production of IFN-γ, efficient cytolysis, and protection from vaccinia virus infection. Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses.

AB - Dendritic cells phagocytose pathogens leading to maturation and cross-presentation on MHC class I. We found that the efficiency of cross-priming in mice after vaccination with biodegradable poly(d,l-lactide-co-glycolide) microspheres (MSs) was enhanced when ovalbumin was coencapsulated together with either a CpG oligonucleotide or polyI:C as compared to co-inoculation of ovalbumin-bearing MS with soluble or separately encapsulated adjuvants. A single immunization with MS containing coencaspsulated CpG and ovalbumin yielded 9% SIINFEKL/H-2Kb tetramer positive CTLs, production of IFN-γ, efficient cytolysis, and protection from vaccinia virus infection. Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses.

KW - Biodegradable microspheres

KW - Cytotoxic T cells

KW - Viral infection

UR - https://www.scopus.com/pages/publications/40249092848

U2 - 10.1016/j.vaccine.2008.01.030

DO - 10.1016/j.vaccine.2008.01.030

M3 - Article

C2 - 18295941

AN - SCOPUS:40249092848

SN - 0264-410X

VL - 26

SP - 1626

EP - 1637

JO - Vaccine

JF - Vaccine

IS - 13

ER -

TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses

Abstract

UN SDGs

Keywords

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