TY - GEN
T1 - Three-dimensional registration of synchrotron radiation-based micro-computed tomography images with advanced laboratory micro-computed tomography data from murine kidney casts
AU - Thalmann, Peter
AU - Hieber, Simone E.
AU - Schulz, Georg
AU - Deyhle, Hans
AU - Kurtcuoglu, Vartan
AU - Olgac, Ufuk
AU - Marmaras, Anastasios
AU - Kuo, Willy
AU - Meyer, Eric P.
AU - Beckmann, Felix
AU - Herzen, Julia
AU - Ehrbar, Stefanie
AU - Müller, Bert
N1 - Publisher Copyright:
© 2014 SPIE.
PY - 2014
Y1 - 2014
N2 - Malfunction of oxygen regulation in kidney and liver may lead to the pathogenesis of chronic diseases. The underlying mechanisms are poorly understood. In kidney, it is hypothesized that renal gas shunting from arteries to veins eliminates excess oxygen. Such shunting is highly dependent on the structure of the renal vascular network. The vascular tree has so far not been quantified under maintenance of its connectivity. Three-dimensional imaging of the vessel tree down to the smallest capillaries, which in mouse model are smaller than 5 μm in diameter, is a challenging task. An established protocol is to use corrosion casts and apply synchrotron radiation-based micro computed tomography (SRμCT), which provides the desired spatial resolution with the necessary contrast. However, SRμCT is expensive and beamtime access is limited. We show here that measurements with a phoenix nanotom®m (General Electric, Wunstorf, Germany) can provide comparable results to those obtained with SRμCT, but for regions with high prevalence of small vessel structures, where the signal to noise level was significantly reduced. For this purpose the nanotom®m measurement was compared with its corresponding measurement acquired at the beamline P05 at PETRA III at DESY, Hamburg, Germany.
AB - Malfunction of oxygen regulation in kidney and liver may lead to the pathogenesis of chronic diseases. The underlying mechanisms are poorly understood. In kidney, it is hypothesized that renal gas shunting from arteries to veins eliminates excess oxygen. Such shunting is highly dependent on the structure of the renal vascular network. The vascular tree has so far not been quantified under maintenance of its connectivity. Three-dimensional imaging of the vessel tree down to the smallest capillaries, which in mouse model are smaller than 5 μm in diameter, is a challenging task. An established protocol is to use corrosion casts and apply synchrotron radiation-based micro computed tomography (SRμCT), which provides the desired spatial resolution with the necessary contrast. However, SRμCT is expensive and beamtime access is limited. We show here that measurements with a phoenix nanotom®m (General Electric, Wunstorf, Germany) can provide comparable results to those obtained with SRμCT, but for regions with high prevalence of small vessel structures, where the signal to noise level was significantly reduced. For this purpose the nanotom®m measurement was compared with its corresponding measurement acquired at the beamline P05 at PETRA III at DESY, Hamburg, Germany.
KW - Bifurcation density
KW - Capillaries
KW - Computed microtomography
KW - Morphology osmium staining
KW - Post mortem imaging
KW - Vascularization
KW - Vessel microstructures
UR - http://www.scopus.com/inward/record.url?scp=84923044948&partnerID=8YFLogxK
U2 - 10.1117/12.2060809
DO - 10.1117/12.2060809
M3 - Conference contribution
AN - SCOPUS:84923044948
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Developments in X-Ray Tomography IX
A2 - Stock, Stuart R.
PB - SPIE
T2 - Developments in X-Ray Tomography IX
Y2 - 18 August 2014 through 20 August 2014
ER -