Three-dimensional registration of synchrotron radiation-based micro-computed tomography images with advanced laboratory micro-computed tomography data from murine kidney casts

Peter Thalmann, Simone E. Hieber, Georg Schulz, Hans Deyhle, Vartan Kurtcuoglu, Ufuk Olgac, Anastasios Marmaras, Willy Kuo, Eric P. Meyer, Felix Beckmann, Julia Herzen, Stefanie Ehrbar, Bert Müller

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Malfunction of oxygen regulation in kidney and liver may lead to the pathogenesis of chronic diseases. The underlying mechanisms are poorly understood. In kidney, it is hypothesized that renal gas shunting from arteries to veins eliminates excess oxygen. Such shunting is highly dependent on the structure of the renal vascular network. The vascular tree has so far not been quantified under maintenance of its connectivity. Three-dimensional imaging of the vessel tree down to the smallest capillaries, which in mouse model are smaller than 5 μm in diameter, is a challenging task. An established protocol is to use corrosion casts and apply synchrotron radiation-based micro computed tomography (SRμCT), which provides the desired spatial resolution with the necessary contrast. However, SRμCT is expensive and beamtime access is limited. We show here that measurements with a phoenix nanotom®m (General Electric, Wunstorf, Germany) can provide comparable results to those obtained with SRμCT, but for regions with high prevalence of small vessel structures, where the signal to noise level was significantly reduced. For this purpose the nanotom®m measurement was compared with its corresponding measurement acquired at the beamline P05 at PETRA III at DESY, Hamburg, Germany.

Original languageEnglish
Title of host publicationDevelopments in X-Ray Tomography IX
EditorsStuart R. Stock
PublisherSPIE
ISBN (Electronic)9781628412390
DOIs
StatePublished - 2014
EventDevelopments in X-Ray Tomography IX - San Diego, United States
Duration: 18 Aug 201420 Aug 2014

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume9212
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceDevelopments in X-Ray Tomography IX
Country/TerritoryUnited States
CitySan Diego
Period18/08/1420/08/14

Keywords

  • Bifurcation density
  • Capillaries
  • Computed microtomography
  • Morphology osmium staining
  • Post mortem imaging
  • Vascularization
  • Vessel microstructures

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