TY - GEN
T1 - Three-dimensional optical tomography of fluorescent proteins in the visible
AU - Zacharakis, Giannis
AU - Ripoll, Jorge
AU - Ishii, Ken
AU - Kambara, Hirokazu
AU - Saeki, Yoshinaga
AU - Weissleder, Ralph
AU - Ntziachristos, Vasilis
N1 - Publisher Copyright:
© 2003 Optical Society of America
PY - 2004
Y1 - 2004
N2 - We present a new modality for tomographic imaging of fluorescence activity in the visible light range. The tomographic method employed a multi-angle, multi-projection illumination scheme from an Ar+ laser emitting at 488nm, while detection was achieved using a highly sensitive CCD camera. Reconstruction is based on novel photon propagation models developed specifically for visible light. We show that the technique can quantitatively detect fluorescence with sub millimeter spatial resolution. We also show that the detection of gene expression (using GFP) is possible in deep tissues quantitatively.
AB - We present a new modality for tomographic imaging of fluorescence activity in the visible light range. The tomographic method employed a multi-angle, multi-projection illumination scheme from an Ar+ laser emitting at 488nm, while detection was achieved using a highly sensitive CCD camera. Reconstruction is based on novel photon propagation models developed specifically for visible light. We show that the technique can quantitatively detect fluorescence with sub millimeter spatial resolution. We also show that the detection of gene expression (using GFP) is possible in deep tissues quantitatively.
UR - https://www.scopus.com/pages/publications/85134590123
M3 - Conference contribution
AN - SCOPUS:85134590123
T3 - Optics InfoBase Conference Papers
BT - Biomedical Topical Meeting, BIOMED 2004
PB - Optica Publishing Group (formerly OSA)
T2 - Biomedical Topical Meeting, BIOMED 2004
Y2 - 14 April 2004 through 17 April 2004
ER -