@inbook{c482f0502c8147a98cb3fe8a54b1be7f,
title = "The use of a laser for correlating light and electron microscopic images in thick tissue specimens",
abstract = "Correlating images between light and electron microscopy is difficult especially in tissue specimens with a substantial z-dimension. To facilitate correlated light and electron microscopy (CLEM) in thick tissue specimens, we describe a basic method of using a femto-pulsed near-infrared laser to burn precise three-dimensional fiducial markers that circumscribe cells or regions of interest for easy identification between imaging methods. This rapid and reliable approach permits traditional fixation and avoids the use of electron-dense labeling methods that can obscure ultrastructural details. The versatility of the technique permits CLEM in a variety of tissue specimens to allow interpretation of highly resolved ultrastructural data in the more macroscopic and potentially dynamic context of light microscopy.",
keywords = "Confocal microscopy, Electron microscopy, In vivo, Multiphoton microscopy, Serial section reconstruction, Technique",
author = "Derron Bishop and Ivana Nikic and Martin Kerschensteiner and Thomas Misgeld",
note = "Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",
year = "2014",
doi = "10.1016/B978-0-12-801075-4.00015-X",
language = "English",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "323--337",
booktitle = "Methods in Cell Biology",
}