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The triple lux-a experiment for BIOLAB/ISS - Combined effects of microgravity and cosmic radiation on the oxidative burst of mammalian macrophageal cells

  • K. Huber
  • , J. Sromicki
  • , B. Hock
  • , O. Ullrich
  • Otto-von-Guericke University
  • University of Zurich

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Phagocytes, the prominent cells of innate immunity, are responsible for the removal of foreign invaders, apoptotic as well as cancer cells. In a flight experiment in the BIOLAB facility on the ISS we will investigate the combined effects of microgravity and cosmic radiation on the oxidative burst, the production of reactive oxygen species (ROS), of the macrophageal cell line NR8383. A chemiluminescence assay (luminol) is used to determine the amount of ROS during phagocytosis of zymosan in a kinetic approach. Ground control experiments for the TRIPLE LUX-A flight experiment on a fast rotating 2D clinostat showed that the selected cell line responds to simulated weightlessness by an increase of ROSproduction.

Original languageEnglish
Title of host publicationProceedings of the Symposium Life in Space for Life on Earth
StatePublished - 2008
EventLife in Space for Life on Earth - Angers, France
Duration: 22 Jun 200827 Jul 2008

Publication series

NameEuropean Space Agency, (Special Publication) ESA SP
Volume663 SP
ISSN (Print)0379-6566

Conference

ConferenceLife in Space for Life on Earth
Country/TerritoryFrance
CityAngers
Period22/06/0827/07/08

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

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