The transcription factor WRKY22 is required during cryo-stress acclimation in Arabidopsis shoot tips

Johanna Stock, Andrea Bräutigam, Michael Melzer, Gerd Patrick Bienert, Boyke Bunk, Manuela Nagel, Jörg Overmann, E. R. Joachim Keller, Hans Peter Mock

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Storage of meristematic tissue at ultra-low temperatures offers a mean to maintain valuable genetic resources from vegetatively reproduced plants. To reveal the biology underlying cryo-stress, shoot tips of the model plant Arabidopsis thaliana were subjected to a standard preservation procedure. A transcriptomic approach was taken to describe the subsequent cellular events which occurred. The cryoprotectant treatment induced the changes in the transcript levels of genes associated with RNA processing and primary metabolism. Explants of a mutant lacking a functional copy of the transcription factor WRKY22 were compromised for recovery. A number of putative downstream targets of WRKY22 were identified, some related to phytohormone-mediated defense, to the osmotic stress response, and to development. There were also alterations in the abundance of transcript produced by genes encoding photosynthesis-related proteins. The wrky22 mutant plants developed an open stomata phenotype in response to their exposure to the cryoprotectant solution. WRKY22 probably regulates a transcriptional network during cryo-stress, linking the explant's defense and osmotic stress responses to changes in its primary metabolism. A model is proposed linking WRKY53 and WRKY70 downstream of the action of WRKY22.

Original languageEnglish
Pages (from-to)4993-5009
Number of pages17
JournalJournal of Experimental Botany
Volume71
Issue number16
DOIs
StatePublished - 1 Aug 2020
Externally publishedYes

Keywords

  • Abiotic stress
  • Cryoprotectant
  • Shoot tip
  • Stomatal closure
  • Transcription factor
  • Transcriptomics
  • Ultra-low temperature

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