The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition

Andreas Lingel; Bernd Simon; Elisa Izaurralde; Michael Sattler

doi:10.1038/sj.embor.7400583

The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition

Andreas Lingel, Bernd Simon, Elisa Izaurralde, Michael Sattler

European Molecular Biology Laboratory Heidelberg

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three α-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (α2/α2′) forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.

Original language	English
Pages (from-to)	1149-1155
Number of pages	7
Journal	EMBO Reports
Volume	6
Issue number	12
DOIs	https://doi.org/10.1038/sj.embor.7400583
State	Published - Dec 2005
Externally published	Yes

Keywords

RNAi
Viral suppressor
dsRNA recognition

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10.1038/sj.embor.7400583

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abstract = "We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three α-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (α2/α2′) forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.",

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AU - Lingel, Andreas

AU - Simon, Bernd

AU - Izaurralde, Elisa

AU - Sattler, Michael

PY - 2005/12

Y1 - 2005/12

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KW - RNAi

KW - Viral suppressor

KW - dsRNA recognition

UR - https://www.scopus.com/pages/publications/28544452860

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DO - 10.1038/sj.embor.7400583

M3 - Article

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JO - EMBO Reports

JF - EMBO Reports

IS - 12

ER -

The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition

Abstract

Keywords

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