The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition

Andreas Lingel; Bernd Simon; Elisa Izaurralde; Michael Sattler

doi:10.1038/sj.embor.7400583

The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition

Andreas Lingel, Bernd Simon, Elisa Izaurralde, Michael Sattler

European Molecular Biology Laboratory Heidelberg

Research output: Contribution to journal › Article › peer-review

113 Scopus citations

Abstract

We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three α-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (α2/α2′) forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.

Original language	English
Pages (from-to)	1149-1155
Number of pages	7
Journal	EMBO Reports
Volume	6
Issue number	12
DOIs	https://doi.org/10.1038/sj.embor.7400583
State	Published - Dec 2005
Externally published	Yes

Keywords

RNAi
Viral suppressor
dsRNA recognition

Access to Document

10.1038/sj.embor.7400583

Cite this

@article{021b000c7337454084d02e2085a728a4,

title = "The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition",

abstract = "We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three α-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (α2/α2′) forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.",

keywords = "RNAi, Viral suppressor, dsRNA recognition",

author = "Andreas Lingel and Bernd Simon and Elisa Izaurralde and Michael Sattler",

year = "2005",

month = dec,

doi = "10.1038/sj.embor.7400583",

language = "English",

volume = "6",

pages = "1149--1155",

journal = "EMBO Reports",

issn = "1469-221X",

publisher = "Wiley-Blackwell",

number = "12",

}

TY - JOUR

T1 - The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition

AU - Lingel, Andreas

AU - Simon, Bernd

AU - Izaurralde, Elisa

AU - Sattler, Michael

PY - 2005/12

Y1 - 2005/12

N2 - We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three α-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (α2/α2′) forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.

AB - We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three α-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (α2/α2′) forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.

KW - RNAi

KW - Viral suppressor

KW - dsRNA recognition

UR - http://www.scopus.com/inward/record.url?scp=28544452860&partnerID=8YFLogxK

U2 - 10.1038/sj.embor.7400583

DO - 10.1038/sj.embor.7400583

M3 - Article

C2 - 16270100

AN - SCOPUS:28544452860

SN - 1469-221X

VL - 6

SP - 1149

EP - 1155

JO - EMBO Reports

JF - EMBO Reports

IS - 12

ER -

The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this