The stiffness of bone marrow cell-knit composites is increased during mechanical load

A. Bruinink, D. Siragusano, G. Ettel, T. Brandsberg, F. Brandsberg, M. Petitmermet, B. Müller, J. Mayer, E. Wintermantel

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


A novel device for mechanical stimulation of primary adult rat bone marrow cells cultured on three-dimensional knitted textiles has been prototyped. A method has been developed ensuring a well-defined, high-density, and reproducible cell seeding on the knitted fabric. After culturing for 18-52 days the cell-knit composites were subjected to uniaxial 2% stretching and relaxation. The frequency was altered between 0.1 Hz (196 min, loading phase) and 0.01 Hz (360 min, resting phase). Identically treated knits without cells exhibited a slight stiffness reduction, whereas the stiffness of knits with cells increased from cycle to cycle. The stiffness increase was found to depend on the duration of the culture period before mechanical loading. Our data suggest that the extracellular matrix deposited by the cells on the knit and intact microtubuli of living cells cause the observed stiffness increase. In comparison to the unstrained static cell-knit composites cell proliferation and bone cell differentiation were reduced by the mechanical load.

Original languageEnglish
Pages (from-to)3169-3178
Number of pages10
Issue number23
StatePublished - 2001
Externally publishedYes


  • Bone marrow
  • Culture
  • Knit
  • Mechanical load
  • Stiffness
  • Strain


Dive into the research topics of 'The stiffness of bone marrow cell-knit composites is increased during mechanical load'. Together they form a unique fingerprint.

Cite this