@article{59b146ada3fc438099b2326ba25ec1e4,
title = "The stem cell population of Arabidopsis shoot meristems is maintained by a regulatory loop between the CLAVATA and WUSCHEL genes",
abstract = "The higher-plant shoot meristem is a dynamic structure whose maintenance depends on the coordination of two antagonistic processes, organ initiation and self-renewal of the stem cell population. In Arabidopsis shoot and floral meristems, the WUSCHEL (WUS) gene is required for stem cell identity, whereas the CLAVATA1, 2, and 3 (CLV) genes promote organ initiation. Our analysis of the interactions between these key regulators indicates that (1) the CLV genes repress WUS at the transcript level and that (2) WUS expression is sufficient to induce meristem cell identity and the expression of the stem cell marker CLV3. Our data suggest that the shoot meristem has properties of a self-regulatory system in which WUS/CLV interactions establish a feedback loop between the stem cells and the underlying organizing center.",
author = "Heiko Schoof and Michael Lenhard and Achim Haecker and Mayer, {Klaus F.X.} and Gerd J{\"u}rgens and Thomas Laux",
note = "Funding Information: We would like to thank Arp Schnittger and the members of the Laux laboratory for helpful comments on the manuscript. We are grateful to Robert Williams and Elliot Meyerowitz for providing the CLV1 upstream region and to David Smyth for providing the ANT upstream region prior to publication. We thank Steven Clark for providing clv seeds, Detlef Weigel for communicating the GUS staining protocol, and Ian Moore for providing the plasmids for the two-component system. This work was supported by grants from the Deutsche Forschungsgemeinschaft to T. L. and stipends from the Konrad-Adenauer-Stiftung (H. S.) and from the Boehringer Ingelheim Fonds (M. L.).",
year = "2000",
month = mar,
day = "17",
doi = "10.1016/S0092-8674(00)80700-X",
language = "English",
volume = "100",
pages = "635--644",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}