Abstract
Porphyrin production by the haemin-independent Haemophilus parainfluenzae in the diagnostic porphyrin test, which determines the X-factor requirement in Haemophilus spp., was analysed quantitatively by applying modern high-performance liquid chromatographic (HPLC) methods. Ion-pair reversed-phase HPLC enabled the simultaneous separation of all porphyrin intermediates and their isomers of haem biosynthesis produced by the bacteria. The pH-dependence of porphyrin production and the respective composition of the porphyrins within the bacterial cells and in the supernate were investigated. A pH optimum of 6.9-8.0 for the production of porphyrins was found and there were marked differences in the porphyrin profiles at different pH values.
| Original language | English |
|---|---|
| Pages (from-to) | 262-267 |
| Number of pages | 6 |
| Journal | Journal of Medical Microbiology |
| Volume | 39 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |