TY - JOUR
T1 - The probability of a horizontal gene transfer from Roundup Ready® soybean to root symbiotic bacteria
T2 - A risk assessment study on the GSF lysimeter station
AU - Wagner, T.
AU - Arango Isaza, L. M.
AU - Grundmann, S.
AU - Dörfler, U.
AU - Schroll, R.
AU - Schloter, M.
AU - Hartmann, A.
AU - Sandermann, H.
AU - Ernst, D.
PY - 2008/4
Y1 - 2008/4
N2 - The gene transfer from glyphosate tolerant soybean to Bradyrhizobium japonicum was evaluated in a free-air lysimeter experiment under natural conditions and increasing selection pressure, to monitor for the probability of horizontal gene transfer (HGT). A large volume lysimeter study that offers conditions comparable to normal farming was conducted in 2004 and 2005 with Roundup Ready® (RR) soybean and Roundup® application according to agricultural practice. Analysis of nodules showed, as expected, the presence of the transgenic 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). However, in bacteroids that were isolated from nodules and then cultivated for several rounds in the presence of high levels of glyphosate, the EPSPS gene could no longer be detected. This indicates no stable HGT transfer of the whole EPSPS gene under field conditions.
AB - The gene transfer from glyphosate tolerant soybean to Bradyrhizobium japonicum was evaluated in a free-air lysimeter experiment under natural conditions and increasing selection pressure, to monitor for the probability of horizontal gene transfer (HGT). A large volume lysimeter study that offers conditions comparable to normal farming was conducted in 2004 and 2005 with Roundup Ready® (RR) soybean and Roundup® application according to agricultural practice. Analysis of nodules showed, as expected, the presence of the transgenic 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). However, in bacteroids that were isolated from nodules and then cultivated for several rounds in the presence of high levels of glyphosate, the EPSPS gene could no longer be detected. This indicates no stable HGT transfer of the whole EPSPS gene under field conditions.
KW - Enolpyruvylshikimate-3-phosphate synthase
KW - Free-air lysimeter
KW - Glyphosate
KW - Horizontal gene transfer
KW - Roundup Ready® soybean
KW - Symbiotic bacteria
UR - http://www.scopus.com/inward/record.url?scp=41549109473&partnerID=8YFLogxK
U2 - 10.1007/s11267-007-9168-0
DO - 10.1007/s11267-007-9168-0
M3 - Article
AN - SCOPUS:41549109473
SN - 1567-7230
VL - 8
SP - 155
EP - 162
JO - Water, Air, and Soil Pollution: Focus
JF - Water, Air, and Soil Pollution: Focus
IS - 2
ER -