The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae

Nina Springer; Wolfgang Ludwig; Wincentry Drozański; Rudolf Amann; Karl Heinz Schleifer

doi:10.1016/0378-1097(92)90403-B

The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae

Nina Springer
, Wolfgang Ludwig
, Wincentry Drozański
, Rudolf Amann
, Karl Heinz Schleifer

TUM Emeriti of Excellence

Technical University of Munich
Maria-Curie Skłodowska University

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

A 16S rRNA gene of the obligate intracellular bacterial parasite Sarcobium lyticum was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was cloned, sequenced and compared with other bacterial 16S rRNA sequences. The analysis revealed that S. lyticum belongs to the gamma subclass of the Proteobacteria and shows the closest relationship to an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. S. lyticum could be detected in situ with a fluorescent oligonucleotide probe by whole cell hybridization.

Original language	English
Pages (from-to)	199-202
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	96
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1097(92)90403-B
State	Published - 15 Sep 1992

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

16S rRNA
Identification, in situ
Intracellular bacterial parasite
Legionella
Phylogeny
Sarcobium lyticum

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10.1016/0378-1097(92)90403-B

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title = "The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae",

abstract = "A 16S rRNA gene of the obligate intracellular bacterial parasite Sarcobium lyticum was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was cloned, sequenced and compared with other bacterial 16S rRNA sequences. The analysis revealed that S. lyticum belongs to the gamma subclass of the Proteobacteria and shows the closest relationship to an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. S. lyticum could be detected in situ with a fluorescent oligonucleotide probe by whole cell hybridization.",

keywords = "16S rRNA, Identification, in situ, Intracellular bacterial parasite, Legionella, Phylogeny, Sarcobium lyticum",

author = "Nina Springer and Wolfgang Ludwig and Wincentry Droza{\'n}ski and Rudolf Amann and Schleifer, \{Karl Heinz\}",

year = "1992",

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doi = "10.1016/0378-1097(92)90403-B",

language = "English",

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pages = "199--202",

journal = "FEMS Microbiology Letters",

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T1 - The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae

AU - Springer, Nina

AU - Ludwig, Wolfgang

AU - Drozański, Wincentry

AU - Amann, Rudolf

AU - Schleifer, Karl Heinz

PY - 1992/9/15

Y1 - 1992/9/15

N2 - A 16S rRNA gene of the obligate intracellular bacterial parasite Sarcobium lyticum was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was cloned, sequenced and compared with other bacterial 16S rRNA sequences. The analysis revealed that S. lyticum belongs to the gamma subclass of the Proteobacteria and shows the closest relationship to an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. S. lyticum could be detected in situ with a fluorescent oligonucleotide probe by whole cell hybridization.

AB - A 16S rRNA gene of the obligate intracellular bacterial parasite Sarcobium lyticum was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was cloned, sequenced and compared with other bacterial 16S rRNA sequences. The analysis revealed that S. lyticum belongs to the gamma subclass of the Proteobacteria and shows the closest relationship to an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. S. lyticum could be detected in situ with a fluorescent oligonucleotide probe by whole cell hybridization.

KW - 16S rRNA

KW - Identification, in situ

KW - Intracellular bacterial parasite

KW - Legionella

KW - Phylogeny

KW - Sarcobium lyticum

UR - https://www.scopus.com/pages/publications/0027120859

U2 - 10.1016/0378-1097(92)90403-B

DO - 10.1016/0378-1097(92)90403-B

M3 - Article

C2 - 1383081

AN - SCOPUS:0027120859

SN - 0378-1097

VL - 96

SP - 199

EP - 202

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae

Abstract

UN SDGs

Keywords

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