The Negatome database: A reference set of non-interacting protein pairs

Pawel Smialowski, Philipp Pagel, Philip Wong, Barbara Brauner, Irmtraud Dunger, Gisela Fobo, Goar Frishman, Corinna Montrone, Thomas Rattei, Dmitrij Frishman, Andreas Ruepp

Research output: Contribution to journalArticlepeer-review

106 Scopus citations


The Negatome is a collection of protein and domain pairs that are unlikely to be engaged in direct physical interactions. The database currently contains experimentally supported non-interacting protein pairs derived from two distinct sources: by manual curation of literature and by analyzing protein complexes with known 3D structure. More stringent lists of non-interacting pairs were derived from these two datasets by excluding interactions detected by high-throughput approaches. Additionally, non-interacting protein domains have been derived from the stringent manual and structural data, respectively. The Negatome is much less biased toward functionally dissimilar proteins than the negative data derived by randomly selecting proteins from different cellular locations. It can be used to evaluate protein and domain interactions from new experiments and improve the training of interaction prediction algorithms. The Negatome database is available at

Original languageEnglish
Article numbergkp1026
Pages (from-to)D540-D544
JournalNucleic Acids Research
Issue numberSUPPL.1
StatePublished - 17 Nov 2009


Dive into the research topics of 'The Negatome database: A reference set of non-interacting protein pairs'. Together they form a unique fingerprint.

Cite this