The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments

Stephen A. Bustin, Vladimir Benes, Jeremy A. Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W. Pfaffl, Gregory L. Shipley, Jo Vandesompele, Carl T. Wittwer

Research output: Contribution to journalArticlepeer-review

11538 Scopus citations

Abstract

BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real- time PCR (qPCR) experiments. The problem is exac- erbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments. CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote con- sistency between laboratories, and increase experimen- tal transparency. MIQE is a set of guidelines that de- scribe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can as- sess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is neces- sary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement. SUMMARY: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.

Original languageEnglish
Pages (from-to)611-622
Number of pages12
JournalClinical Chemistry
Volume55
Issue number4
DOIs
StatePublished - 1 Apr 2009

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