The microstructure of mandibular bone grafts and three-dimensional cell clusters

Sarper Gürel, Corinne Unold, Hans Deyhle, Georg Schulz, Sebastian Kühl, Belma Saldamli, Jutta Tübel, Rainer Burgkart, Felix Beckmann, Bert Müller

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

During the last decade, several tissues and biomaterials for medical applications in replacing bony tissues have been developed. Three-dimensional cell clusters and mandibular bone grafts are two distinct examples of these developments. The characterization of the complex three-dimensional structures, however, is still mainly restricted on the twodimensional analysis of histological slices. The present paper examines the quantitative analysis of mandibular bone grafts and three-dimensional cell clusters on the basis of synchrotron radiation-based micro computed tomography measurements. An automated search of pre-defined microstructures through component labeling is applied to the real datasets in order to identify features that reside independently from other components. The examples demonstrate three levels of complexity: rather large pieces of bone augmentation material that touch each other, individual adipocytes difficult to automatically segment in a wet cluster and osmium-stained adipocyte exhibiting higher X-ray absorption than the surrounding tissue. Although the structures of interest such as the cells can be labeled, de-clustering of the components requires the incorporation of erosion and dilation algorithms.

Original languageEnglish
Title of host publicationDevelopments in X-Ray Tomography VII
DOIs
StatePublished - 2010
EventDevelopments in X-Ray Tomography VII - San Diego, CA, United States
Duration: 2 Aug 20105 Aug 2010

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7804
ISSN (Print)0277-786X

Conference

ConferenceDevelopments in X-Ray Tomography VII
Country/TerritoryUnited States
CitySan Diego, CA
Period2/08/105/08/10

Keywords

  • adipocyte
  • bone graft
  • component labeling
  • micro computed tomography
  • socket preservation
  • synchrotron radiation
  • three-dimensional cell cluster

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