The loci for parathyroid hormone and β-globin are closely linked and map to chromosome 15 in cattle

R. Fries; R. Hediger; G. Stranzinger

doi:10.1016/0888-7543(88)90120-6

The loci for parathyroid hormone and β-globin are closely linked and map to chromosome 15 in cattle

R. Fries, R. Hediger, G. Stranzinger

ETH Zurich

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

We report linkage of the loci for β-globin (HBB) and parathyroid hormone (PTH) in cattle and the assignment of both loci to the bovine chromosome region 15q13-q23. Linkage was analyzed in a family of paternal half-sibs by the use of restriction fragment length polymorphisms detected with bovine probes derived from the HBB and PTH genes. The HBB polymorphism was detected by digestion with restriction endonuclease HindIII and the PTH polymorphism with MspI. The maximum lod score for linkage of PTH with HBB was z = 4.52 at θ,= 0, suggesting very close linkage of the two loci. The finding of the PTH HBB linkage is corroborated by the physical assignment of both loci to the region 15q13-q23 by in situ hybridization with bovine genomic probes derived from PTH and HBB, respectively. Since HBB and PTH are syntenic in man and mouse, these results in cattle represent another example of conservation of synteny in the evolution of mammalian chromosomes.

Original language	English
Pages (from-to)	302-307
Number of pages	6
Journal	Genomics
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/0888-7543(88)90120-6
State	Published - Nov 1988
Externally published	Yes

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@article{066f509be19448b4bcc860f02c674e69,

title = "The loci for parathyroid hormone and β-globin are closely linked and map to chromosome 15 in cattle",

abstract = "We report linkage of the loci for β-globin (HBB) and parathyroid hormone (PTH) in cattle and the assignment of both loci to the bovine chromosome region 15q13-q23. Linkage was analyzed in a family of paternal half-sibs by the use of restriction fragment length polymorphisms detected with bovine probes derived from the HBB and PTH genes. The HBB polymorphism was detected by digestion with restriction endonuclease HindIII and the PTH polymorphism with MspI. The maximum lod score for linkage of PTH with HBB was z = 4.52 at θ,= 0, suggesting very close linkage of the two loci. The finding of the PTH HBB linkage is corroborated by the physical assignment of both loci to the region 15q13-q23 by in situ hybridization with bovine genomic probes derived from PTH and HBB, respectively. Since HBB and PTH are syntenic in man and mouse, these results in cattle represent another example of conservation of synteny in the evolution of mammalian chromosomes.",

author = "R. Fries and R. Hediger and G. Stranzinger",

note = "Funding Information: ACKNOWLEDGMENTS We acknowledge the excellent technical assistance of L. Zumbiihl, K. Wiithrich, and A. Eggen, and we thank Professor N. Fecbheimer for critically reading the manuscript. We also thank Dr. B. Kernper and Dr. C. H. Duncan for making the cloned sequences available, the Swiss Association for Artificial Insemination for collecting blood samples of bulls, and the Swiss Simmental Breeding Association for providing us with data on the investigated family. This work was supported by grants from the Swiss National Science Foundation and from the Federal Institute of Technology.",

year = "1988",

month = nov,

doi = "10.1016/0888-7543(88)90120-6",

language = "English",

volume = "3",

pages = "302--307",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "4",

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T1 - The loci for parathyroid hormone and β-globin are closely linked and map to chromosome 15 in cattle

AU - Fries, R.

AU - Hediger, R.

AU - Stranzinger, G.

N1 - Funding Information: ACKNOWLEDGMENTS We acknowledge the excellent technical assistance of L. Zumbiihl, K. Wiithrich, and A. Eggen, and we thank Professor N. Fecbheimer for critically reading the manuscript. We also thank Dr. B. Kernper and Dr. C. H. Duncan for making the cloned sequences available, the Swiss Association for Artificial Insemination for collecting blood samples of bulls, and the Swiss Simmental Breeding Association for providing us with data on the investigated family. This work was supported by grants from the Swiss National Science Foundation and from the Federal Institute of Technology.

PY - 1988/11

Y1 - 1988/11

N2 - We report linkage of the loci for β-globin (HBB) and parathyroid hormone (PTH) in cattle and the assignment of both loci to the bovine chromosome region 15q13-q23. Linkage was analyzed in a family of paternal half-sibs by the use of restriction fragment length polymorphisms detected with bovine probes derived from the HBB and PTH genes. The HBB polymorphism was detected by digestion with restriction endonuclease HindIII and the PTH polymorphism with MspI. The maximum lod score for linkage of PTH with HBB was z = 4.52 at θ,= 0, suggesting very close linkage of the two loci. The finding of the PTH HBB linkage is corroborated by the physical assignment of both loci to the region 15q13-q23 by in situ hybridization with bovine genomic probes derived from PTH and HBB, respectively. Since HBB and PTH are syntenic in man and mouse, these results in cattle represent another example of conservation of synteny in the evolution of mammalian chromosomes.

AB - We report linkage of the loci for β-globin (HBB) and parathyroid hormone (PTH) in cattle and the assignment of both loci to the bovine chromosome region 15q13-q23. Linkage was analyzed in a family of paternal half-sibs by the use of restriction fragment length polymorphisms detected with bovine probes derived from the HBB and PTH genes. The HBB polymorphism was detected by digestion with restriction endonuclease HindIII and the PTH polymorphism with MspI. The maximum lod score for linkage of PTH with HBB was z = 4.52 at θ,= 0, suggesting very close linkage of the two loci. The finding of the PTH HBB linkage is corroborated by the physical assignment of both loci to the region 15q13-q23 by in situ hybridization with bovine genomic probes derived from PTH and HBB, respectively. Since HBB and PTH are syntenic in man and mouse, these results in cattle represent another example of conservation of synteny in the evolution of mammalian chromosomes.

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DO - 10.1016/0888-7543(88)90120-6

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AN - SCOPUS:0024115694

SN - 0888-7543

VL - 3

SP - 302

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JO - Genomics

JF - Genomics

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ER -

The loci for parathyroid hormone and β-globin are closely linked and map to chromosome 15 in cattle

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