The light-harvesting core-complex and the B820-subunit from Rhodopseudomonas marina. Part I. Purification and characterisation

Rainer U. Meckenstock, René A. Brunisholz, Herbert Zuber

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Abstract

The BChla-containing B880-complex (core-complex) of Rhodopseudomonas marina (Rhodospirillaceae) was isolated with a new purification method. The isolation of the B880-complex was performed by solubilisation of the photosynthetic membranes with the detergent LDAO and subsequent fractionated ammonium-sulfate precipitation with about 50% recovery. The B880-complex retained its original spectral properties as revealed with absorption, fluorescence and circular dichroism spectroscopy. Furthermore, we dissociated the B880-complex with the detergent n-octyl-β-glucoside (OG) and purified the developed subcomplex by the method of Miller et al. [1], which showed an absorption maximum at 820 nm (B820). The α- to β-polypeptide ratio and the α- or β-polypeptide to BChla ratio, respectively, were estimated to be 1:1 in both complexes. The molecular weights of the B880 and the B820-complexes, determined by gel filtration chromatography, were 181 and 32 kDa, respectively. Thus, it appears that the B880-complex of RP. marina consists of 24 polypeptides and the B820-complex of four polypeptides. Six B820-complexes or possible subunits could form the B880-complex. On the basis of these data we propose a model for the structure or BChla containing core-complexes.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalFEBS Letters
Volume311
Issue number2
DOIs
StatePublished - 19 Oct 1992
Externally publishedYes

Keywords

  • B820
  • B880
  • Bacteriochlorophyll α
  • Light-harvesting complex
  • Non-sulfur purple bacteria
  • Rhodospirillaceae

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