The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli. A mutagenesis study

N. Meier, H. U. Göringer, B. Kleuvers, U. Scheibe, J. Eberle, C. Szymkowiak, M. Zacharias, R. Wagner

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of denned nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalFEBS Letters
Volume204
Issue number1
DOIs
StatePublished - 11 Aug 1986
Externally publishedYes

Keywords

  • Mutagenesis
  • Protein-RNA interaction
  • Subunit association
  • ribosomal RNA
  • tRNA binding

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