The gene LEO1 on yeast chromosome XV encodes a non-essential, extremely hydrophilic protein

Viktor Magdolen; Peter Lang; Günter Mages; Hannes Hermann; Wolfhard Bandlow

doi:10.1016/0167-4781(94)90013-2

The gene LEO1 on yeast chromosome XV encodes a non-essential, extremely hydrophilic protein

Viktor Magdolen
, Peter Lang
, Günter Mages
, Hannes Hermann
, Wolfhard Bandlow

Department of Gynecology

Institut für Genetik and Mikrobiologie

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A 5.6 kbp segment of DNA from Saccharomyces cerevisiae chromosome XV has been isolated and sequenced. Genetic and nucleotide sequence analyses revealed that this region is closely linked to the ADE2 marker on chromosome XV and densely packed with genetic information. We show the gene organization of the entire region and report the nucleotide sequence of the gene, LEO1, which occurs in single copy in the haploid genome. The deduced amino acid sequence specifies an extremely hydrophilic protein with pronounced domain structure (molecular mass 53.9 kDa). The gene is constitutively expressed at a low level and is non-essential, as indicated by the absence of a phenotype from gene disruption mutants.

Original language	English
Pages (from-to)	205-209
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1218
Issue number	2
DOIs	https://doi.org/10.1016/0167-4781(94)90013-2
State	Published - 21 Jun 1994

Keywords

(S. cerevisiae)
Acidic protein
Chromosome XV
Gene organization
Nucleotide sequence

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10.1016/0167-4781(94)90013-2

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title = "The gene LEO1 on yeast chromosome XV encodes a non-essential, extremely hydrophilic protein",

abstract = "A 5.6 kbp segment of DNA from Saccharomyces cerevisiae chromosome XV has been isolated and sequenced. Genetic and nucleotide sequence analyses revealed that this region is closely linked to the ADE2 marker on chromosome XV and densely packed with genetic information. We show the gene organization of the entire region and report the nucleotide sequence of the gene, LEO1, which occurs in single copy in the haploid genome. The deduced amino acid sequence specifies an extremely hydrophilic protein with pronounced domain structure (molecular mass 53.9 kDa). The gene is constitutively expressed at a low level and is non-essential, as indicated by the absence of a phenotype from gene disruption mutants.",

keywords = "(S. cerevisiae), Acidic protein, Chromosome XV, Gene organization, Nucleotide sequence",

author = "Viktor Magdolen and Peter Lang and G{\"u}nter Mages and Hannes Hermann and Wolfhard Bandlow",

note = "Funding Information: We thank G. Strobel and C. Hardtke for excellent technical assistence. The work was aided by a grant of the Deutsche Forschungsgemeinschaft to W.B. within SFB 190.",

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T1 - The gene LEO1 on yeast chromosome XV encodes a non-essential, extremely hydrophilic protein

AU - Magdolen, Viktor

AU - Lang, Peter

AU - Mages, Günter

AU - Hermann, Hannes

AU - Bandlow, Wolfhard

N1 - Funding Information: We thank G. Strobel and C. Hardtke for excellent technical assistence. The work was aided by a grant of the Deutsche Forschungsgemeinschaft to W.B. within SFB 190.

PY - 1994/6/21

Y1 - 1994/6/21

N2 - A 5.6 kbp segment of DNA from Saccharomyces cerevisiae chromosome XV has been isolated and sequenced. Genetic and nucleotide sequence analyses revealed that this region is closely linked to the ADE2 marker on chromosome XV and densely packed with genetic information. We show the gene organization of the entire region and report the nucleotide sequence of the gene, LEO1, which occurs in single copy in the haploid genome. The deduced amino acid sequence specifies an extremely hydrophilic protein with pronounced domain structure (molecular mass 53.9 kDa). The gene is constitutively expressed at a low level and is non-essential, as indicated by the absence of a phenotype from gene disruption mutants.

AB - A 5.6 kbp segment of DNA from Saccharomyces cerevisiae chromosome XV has been isolated and sequenced. Genetic and nucleotide sequence analyses revealed that this region is closely linked to the ADE2 marker on chromosome XV and densely packed with genetic information. We show the gene organization of the entire region and report the nucleotide sequence of the gene, LEO1, which occurs in single copy in the haploid genome. The deduced amino acid sequence specifies an extremely hydrophilic protein with pronounced domain structure (molecular mass 53.9 kDa). The gene is constitutively expressed at a low level and is non-essential, as indicated by the absence of a phenotype from gene disruption mutants.

KW - (S. cerevisiae)

KW - Acidic protein

KW - Chromosome XV

KW - Gene organization

KW - Nucleotide sequence

UR - https://www.scopus.com/pages/publications/0028321543

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DO - 10.1016/0167-4781(94)90013-2

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SN - 0167-4781

VL - 1218

SP - 205

EP - 209

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 2

ER -

The gene LEO1 on yeast chromosome XV encodes a non-essential, extremely hydrophilic protein

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Keywords

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